Ovarian transplantation for fertility preservation in a sheep model: can follicle loss be prevented by antiapoptotic sphingosine-1-phosphate administration?
Ovarian transplantation for fertility preservation in a sheep model: can follicle loss be prevented by antiapoptotic sphingosine-1-phosphate administration?
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DOI：
10.3109/09513590903159524
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发表时间：
2009-01-01
影响因子：
2
通讯作者：
Denschlag, Dominik
中科院分区：
文献类型：
--
作者：
Hancke, Katharina;Walker, Elisabeth;Denschlag, Dominik
目标。本研究的目的是探讨鞘氨醇-1-磷酸（S1 P），一种凋亡抑制剂，是否能够保护卵巢移植中的卵泡免受缺氧诱导的细胞死亡。在四只绵羊中，两个卵巢都被切除，并立即移植回腹壁。其中2例在解剖卵巢皮质后，通过注射和孵育局部用S1 P处理一侧卵巢。对侧卵巢的绵羊和其余绵羊的两个卵巢作为对照。2周后，对所有移植物进行组织学评价。尽管移植后凋亡细胞的平均数量与新鲜组织相比有所增加，但我们无法证明S1 P处理组织中卵泡密度的显著差异（每个视野的平均卵泡数量：0.21 vs. 0.23，p = 0.909）。细胞凋亡似乎在卵巢移植过程中的卵泡损失中发挥作用;然而，在我们的研究中，局部应用S1 P并不能阻止皮质移植物中缺氧诱导的原始/初级卵泡细胞死亡。
Aims. The aim of this study was to investigate whether sphingosine-1-phosphate (S1P), an apoptosis-inhibitor would be able to protect ovarian follicles from hypoxia-induced cell death in ovarian transplantation.Methods. In four sheep, both ovaries were removed and immediately transplanted back into the abdominal wall. In two of them, one ovary was treated with S1P locally by injection and incubation after dissecting the ovarian cortex. The contra lateral ovary of both sheep and both ovaries of the remaining sheep served as controls. After 2 weeks, all grafts were evaluated histologically.Results. Although the mean number of apoptotic cells were increased after grafting compared with fresh tissue, we were not able to demonstrate a significant difference regarding the follicular density in tissue with S1P treatment (mean number of follicles per field of view: 0.21 vs. 0.23, p = 0.909).Conclusion. Apoptosis seems to play a role in follicular loss during ovarian transplantation; however, local application of S1P does not prevent primordial/primary follicles from hypoxia-induced cell death in cortical grafts in our study.