Frequency and distribution of chromosome abnormalities in human oocytes
Frequency and distribution of chromosome abnormalities in human oocytes
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DOI：
10.1159/000086889
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发表时间：
2005-01-01
影响因子：
1.7
通讯作者：
Verlinsky, Y
中科院分区：
文献类型：
--
作者：
Kuliev, A;Cieslak, J;Verlinsky, Y
此前的研究表明，由于减数分裂I和II的错误，来自高龄IVF患者的卵母细胞中有一半以上是非整倍体。本文进一步证实，用13、16、18、21和22号染色体特异的荧光探针检测的卵母细胞中有61.8%是异常的，主要是染色单体错误，这是结果胚胎中非整倍体的主要来源。减数分裂I期错误的卵母细胞中，几乎有一半(49.3%)容易发生II期连续减数分裂错误，这可能导致30.8%的卵母细胞发生非整倍体挽救。检测到的非整倍体中有一半(49.8%)是复杂的，涉及两条或两条以上的染色体，或在两次减数分裂中都有相同的染色体。单个染色体的非整倍体率不同，21号和22号染色体错误的发生率较高。个体染色体的非整倍体起源也不是随机的，16号和22号染色体错误更多地起源于减数分裂II，18，13和21号染色体错误发生在减数分裂I，而且不仅与总的非整倍体频率有关，而且对于每个染色体错误，非整倍体起源于减数分裂I，减数分裂II，减数分裂I和减数分裂II错误，以及不同类型的非整倍体。这些数据进一步表明，卵母细胞非整倍体检测在检测和避免移植来自非整倍体卵母细胞的胚胎方面具有实际意义，这对高龄IVF患者的妊娠结局有重要意义。版权所有c 2005 S.Karger AG，巴塞尔。
It was previously shown that more than half of the human oocytes obtained from IVF patients of advanced reproductive age are aneuploid, due to meiosis I and meiosis II errors. The present paper further confirms that 61.8% of the oocytes tested by fluorescent probes specific for chromosomes 13, 16, 18, 21 and 22 are abnormal, representing predominantly chromatid errors, which are the major source of aneuploidy in the resulting embryos. Almost half of the oocytes with meiosis I errors (49.3%) are prone to sequential meiosis II errors, which may lead to aneuploidy rescue in 30.8% of the cases. Half of the detected aneuploidies (49.8%) are of complex nature with involvement of two or more chromosomes, or the same chromosome in both meiotic divisions. The aneuploidy rates for individual chromosomes are different, with a higher prevalence of chromosome 21 and 22 errors. The origin of aneuploidy for the individual chromosomes is also not random, with chromosome 16 and 22 errors originating more frequently in meiosis II, and chromosome 18, 13 and 21 errors in meiosis I. There is an age dependence not only for the overall frequency of aneuploidies, but also for each chromosome error, aneuploidies originating from meiosis I, meiosis II, and both meiosis I and meiosis II errors, as well as for different types of aneuploidies. The data further suggest the practical relevance of oocyte aneuploidy testing for detection and avoidance from transfer of the embryos deriving from aneuploid oocytes, which should contribute significantly to the pregnancy outcomes of IVF patients of advanced reproduction age. Copyright c 2005 S. Karger AG, Basel.