BmCHSA-2b, a Lepidoptera specific alternative splicing variant of epidermal chitin synthase, is required for pupal wing development in Bombyx mori.
BmCHSA-2b, a Lepidoptera specific alternative splicing variant of epidermal chitin synthase, is required for pupal wing development in Bombyx mori.
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DOI：
10.1016/j.ibmb.2017.06.017
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发表时间：
2017-08
影响因子：
3.8
通讯作者：
Guanfeng Xu;Jie Zhang;H. Lyu;Jia Liu;Yang Ding;Q. Feng;Q. Song;Sichun Zheng
中科院分区：
文献类型：
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作者：
Guanfeng Xu;Jie Zhang;H. Lyu;Jia Liu;Yang Ding;Q. Feng;Q. Song;Sichun Zheng
Insect chitin synthase A (CHSA) is an epidermis-specific enzyme that plays an essential role in insect development. In this study, the function and regulation ofCHSA-2b,an alternative splicing variant ofBombxymoriCHSAthat is discovered only in Lepidopteran insects, were investigated. Analysis of mRNA level showed thatBmCHSA-2bwas responsive to 20-hydroxyecdysone (20E) in pupal wing unlikeBmCHSA-2a,which shares almost the identical sequence asBmCHSA-2bexcept the first 31 amino acids, suggesting that the expression of these two alternative splicing variants is driven by different promoters ofCHSAgene. Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) analysis showed thatBmCHSA-2bwas up-regulated in the wing of mid-pupa unlikeBmCHSA-2a,which was up-regulated in epidermis and wing disc at the beginning and end of pupal stage. Further analysis reveals that the up-regulations ofBmCHSA-2aandBmCHSA-2bin pupal wing were consistent with the increase of chitin content and wing area at the same stages, respectively. Furthermore, the higher transcription level ofBmCHSA-2bin the mid-pupal wing of male than that in female was consistent with the chitin content of pupal wing between genders. Injection of double-stranded RNAs ofBmCHSA-2bresulted in the decrease in the area and chitin content of the wing, and irregular and crimpled vein. All these results together suggest thatB. morievolves an extra promoter inCHSAgene to activateBmCHSA-2bexpression in the wing of mid-pupal stage in response to 20E, andBmCHSA-2bis required for the wing development in the mid-pupa ofB. mori.
