Ultrastructural effects of Clostridium difficile toxin B on smooth muscle cells and fibroblasts.
Ultrastructural effects of Clostridium difficile toxin B on smooth muscle cells and fibroblasts.
复制标题
艰难梭菌毒素 B 对平滑肌细胞和成纤维细胞的超微结构影响。
DOI：
10.1016/0014-4827(83)90163-5
复制
发表时间：
1983
影响因子：
3.7
通讯作者：
LaMont,T
中科院分区：
文献类型：
--
作者：
Wedel,N;Toselli,P;Pothoulakis,C;Faris,B;Oliver,P;Franzblau,C;LaMont,T
研究了艰难梭菌毒素 B 导致培养细胞变圆的机制。用部分纯化或纯化的毒素 B 处理培养的人肺成纤维细胞和兔主动脉平滑肌细胞，并通过光学和透射电子显微镜 (TEM) 进行监测。两种制剂都会导致细胞逐渐变圆，这与含肌动蛋白的肌丝束的解体有关。细肌丝变得支离破碎并最终消失（24 小时后），致密体变得更加突出，而所有其他细胞器似乎不受影响。
The mechanism by whichClostridium difficiletoxin B causes cells in culture to round was investigated. Cultured human lung fibroblasts and rabbit aortic smooth muscle cells were treated with partially purified or purified toxin B and monitored by light and transmission electron microscopy (TEM). Both preparations caused progressive cell rounding which correlated with disorganization of actin-containing myofilament bundles. Thin myofilaments became fragmented and finally disappeared (after 24 h) and dense bodies became more prominent, while all other organelles appeared unaffected.