Continuous dielectrophoretic size-based particle sorting
Continuous dielectrophoretic size-based particle sorting
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DOI：
10.1021/ac0601314
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发表时间：
2006-07-15
影响因子：
7.4
通讯作者：
Jensen, Klavs F.
中科院分区：
文献类型：
--
作者：
Kralj, Jason G.;Lis, Michael T. W.;Jensen, Klavs F.
基于尺寸的连续流介电电泳（DEP）颗粒分离在微流体装置中被证明。悬浮在中性浮力水溶液中的聚苯乙烯微球被用作模型粒子来研究由软光刻微通道内的倾斜的、平面的、叉指状的电极阵列引起的DEP。来自倾斜电极的电场梯度在颗粒上施加净横向力分量，使它们在通道上“棘轮”。在装置的长度上，根据流体动力学阻力和DEP力的平衡，较大颗粒比较小颗粒偏转更多。因此，含有不同尺寸颗粒的流动聚焦颗粒悬浮液随着珠粒流动而分级，并沿装置的长度向下分离。球形颗粒的流动行为建模，和总的横向颗粒位移的微流体装置预测四阶尺寸和电压和二阶逆流速依赖性。该模型的实验验证的范围内的流速，颗粒尺寸和电场强度。
Continuous-flow dielectrophoretic (DEP) particle separation based on size is demonstrated in a microfluidic device. Polystyrene microspheres suspended in a neutrally buoyant aqueous solution are used as model particles to study DEP induced by an array of slanted, planar, interdigitated electrodes inside of a soft-lithography microchannel. The E-field gradients from the slanted electrodes impart a net transverse force component on the particles that causes them to "ratchet" across the channel. Over the length of the device, larger particles are deflected more than smaller particles according to the balance of hydrodynamic drag and DEP forces. Consequently, a flow-focused particle suspension containing different-sized particles is fractionated as the beads flow and separate down the length of the device. The flow behavior of spherical particles is modeled, and the total transverse particle displacement in the microfluidic device predicts fourth-order size and voltage and second-order inverse flow rate dependences. The model is verified experimentally for a range of flow rates, particle sizes, and E-field strengths.