A 2,7-carbazole-based dicationic salt for fluorescence detection of nucleic acids and two-photon fluorescence imaging of RNA in nucleoli and cytoplasm.
A 2,7-carbazole-based dicationic salt for fluorescence detection of nucleic acids and two-photon fluorescence imaging of RNA in nucleoli and cytoplasm.
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DOI：
10.1039/c1ob05123g
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发表时间：
2011-05
影响因子：
3.2
通讯作者：
Xin Liu;Yuming Sun;Yuanhong Zhang;Fang Miao;Guancong Wang;Hongshi Zhao;Xiaoqiang Yu;Hong Liu-
中科院分区：
文献类型：
--
作者：
Xin Liu;Yuming Sun;Yuanhong Zhang;Fang Miao;Guancong Wang;Hongshi Zhao;Xiaoqiang Yu;Hong Liu-
本文报道了一种新的咔唑衍生双阳离子化合物2，7-双（1-羟乙基-4-乙烯基吡啶碘）-N-乙基咔唑（2，7 - 9 E-BHVC），它在核酸中具有较大的双光子吸收截面。此外，它具有在双光子荧光显微镜中对核仁和细胞质中的RNA进行成像的潜力，并且与商业荧光核酸染料DAPI具有良好的复染相容性。
A new carbazole-derived dicationic compound, namely 2,7-bis(1-hydroxyethyl-4-vinylpyridinium iodine)-N-ethylcarbazole (2,7-9E-BHVC), with a large two-photon action absorption cross section in nucleic acids has been obtained. Moreover, it possesses the potential of imaging RNA in nucleoli and cytoplasm in two-photon fluorescence microscopy and exhibits good counterstain compatibility with the commercial fluorescent nucleic dye DAPI.