Variability in Microbial Communities in Black Smoker Chimneys at the NW Caldera Vent Field, Brothers Volcano, Kermadec Arc
Variability in Microbial Communities in Black Smoker Chimneys at the NW Caldera Vent Field, Brothers Volcano, Kermadec Arc
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DOI：
10.1080/01490450903304949
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发表时间：
2009-11
影响因子：
2.3
通讯作者：
K. Takai;T. Nunoura;K. Horikoshi;T. Shibuya;K. Nakamura;K. Nakamura;Yohey Suzuki;M. Stott;G. Massoth;B. Christenson;C. E. J. deRonde;D. Butterfield;D. Butterfield;J. Ishibashi;J. Lupton;L. Evans
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文献类型：
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作者：
K. Takai;T. Nunoura;K. Horikoshi;T. Shibuya;K. Nakamura;K. Nakamura;Yohey Suzuki;M. Stott;G. Massoth;B. Christenson;C. E. J. deRonde;D. Butterfield;D. Butterfield;J. Ishibashi;J. Lupton;L. Evans
克马德克兄弟火山西北火山口喷口场的黑烟囱结构中的微生物群落通过使用依赖于培养物和独立于培养物的技术进行了表征。由端元盐度和气体含量给出的热液喷口流体化学性质在西北火山口区域的黑烟喷发点之间有所不同，这表明流体化学性质可能存在相分离控制的变化。从典型的贫氯和富氯热液喷口收集的烟囱结构用于微生物研究。 16S rRNA 基因克隆分析表明，古细菌 rRNA 基因群落在任何单个烟囱的内部和外部亚结构内都是相似的，甚至在具有贫 Cl 或富 Cl 热液排放的烟囱之间也是如此。相比之下，在含有贫氯或富含氯液体的烟囱之间，细菌 rRNA 基因群落有所不同。培养分析表明，烟囱中各种微生物成分的活菌计数存在显着差异，特别是 H 2 - 和/或 S- 氧化化能微生物，例如 Persephonella 属和 Sulfurimonas 属。烟囱之间培养的微生物群落结构所显示的差异可能与烟囱结构排出的热液的不同化学性质有关，并且可能与喷口下方海底环境的差异有关，特别是在考虑不同的气体输入和碳源时。对迄今为止从包括本研究在内的各种深海热液田研究的烟囱结构中的烟囱结构中培养的微生物种群的模式进行了比较。结果表明，Brothers NW 破火山口喷口场和 Valu Fa Ridge 的 Mariner 喷口场在地理和地质上不同的热液场之间，富含气体热液烟囱的模式非常相似。
Microbial communities in black smoker chimney structures at the NW caldera vent field of the Brothers volcano, Kermadec arc were characterized by using both culture-dependent and -independent techniques. The hydrothermal vent fluid chemistry, as given by end-member salinities and gas contents, differ among the black smoker sites of the NW caldera field, indicating probable phase-separation-controlled variability in the fluid chemistry. Chimney structures collected from typical Cl-depleted and Cl-enriched hydrothermal fluid vents were used for the microbiological investigation. The 16S rRNA gene clone analysis showed that the archaeal rRNA gene communities were similar within interior and exterior substructures of any single chimneys, and even between chimneys having either Cl-depleted or Cl-enriched hydrothermal emissions. By contrast, the bacterial rRNA gene communities varied between chimneys hosting Cl-depleted or Cl-enriched fluids. Cultivation analysis showed significant variation in the viable counts of various microbial components among the chimneys, particularly of H 2 - and/or S-oxidizing chemolithotrophs such as the genera Persephonella and Sulfurimonas. The difference shown by the cultured microbial community structures between the chimneys may be related to the different chemistries of hydrothermal fluids being expelled by the chimney structures, and possibly differences in the subseafloor environments beneath the vent sites, especially when considering different gas inputs and carbon sources. The patterns in cultivated microbial populations in the chimney structures were compared among the chimney structures studied so far from various deep-sea hydrothermal fields including this study. It shows that the patterns from the gas-rich hydrothermal fluid chimneys are quite similar between the geographically and geologically different hydrothermal fields of the Brothers NW caldera vent field, and the Mariner vent field of the Valu Fa Ridge.