Effects of garcinol and guttiferone K isolated from Garcinia cambogia on oxidative/nitrative modifications in blood platelets and plasma
Effects of garcinol and guttiferone K isolated from Garcinia cambogia on oxidative/nitrative modifications in blood platelets and plasma
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DOI：
10.3109/09537100903165182
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发表时间：
2009-01-01
期刊：
影响因子：
3.3
通讯作者：
Wachowicz, Barbara
中科院分区：
文献类型：
--
作者：
Kolodziejczyk, Joanna;Masullo, Milena;Wachowicz, Barbara
Garcinol和guttiferone K，两个聚异戊二烯化的二苯甲酮发生一种食品植物，称为藤黄果，对氧化/硝化蛋白质损伤（由参数，如蛋白质羰基和硝基酪氨酸残基的水平确定）在人血小板和血浆处理后，过氧亚硝酸盐（ONOO-）的影响进行了研究。我们还研究了Garcinol和guttiferone K对ONOO-（100 μ M）诱导的血小板和血浆脂质过氧化反应的影响。暴露的血小板或血浆过氧亚硝酸盐（100 μ M）导致的羰基和硝基酪氨酸残基在蛋白质中的水平增加，和增加的脂质过氧化测定的水平的硫代巴比妥酸反应物种（TBARS）。在加辛和guttiferone K（0.1-25 μ g/ml）的存在下，观察到血浆和血小板蛋白中羰基形成的明显减少以及由100 μ M过氧亚硝酸盐引起的TBARS的减少。然而，Garcinol和guttiferone K没有抑制过亚硝酸盐诱导的血浆和血小板蛋白硝化。存在于人类饮食中的聚异戊二烯化二苯甲酮，如Garcinol或guttiferone K，在体外对脂质和蛋白质氧化具有保护作用，并且可能在体内具有一些有希望的作用，因为它们在体外测试模型中是良好的抗氧化剂。Garcinol和guttiferone K也可以用作对抗与氧化应激相关的疾病的保护因子。
The effects of garcinol and guttiferone K, two polyisoprenylated benzophenones occurring a food plant called Garcinia cambogia, on oxidative/nitrative protein damage (determined by parameters such as levels of protein carbonyl groups and nitrotyrosine residues) in human blood platelets and plasma after treatment with peroxynitrite (ONOO-) were studied in vitro. We also investigated the effects of garcinol and guttiferone K on lipid peroxidation in blood platelets and plasma induced by ONOO- (100 mu M). Exposure of blood platelets or plasma to peroxynitrite (100 mu M) resulted in an increased level of carbonyl groups and nitrotyrosine residues in proteins, and an increase of lipid peroxidation measured by the level of thiobarbituric acid reactive species (TBARS). In the presence of garcinol and guttiferone K (0.1-25 mu g/ml), a distinct reduction in the formation of carbonyl groups in plasma and platelet proteins together with the decrease of TBARS caused by 100 mu M peroxynitrite, was observed. However, garcinol and guttiferone K did not inhibit plasma and platelet protein nitration induced by peroxynitrite. Polyisoprenylated benzophenones present in human diet such as garcinol or guttiferone K in vitro have protective effects against lipid and protein oxidation and may have some promising effects in vivo because they are good antioxidants in the tested models in vitro. Garcinol and guttiferone K can be also useful as protecting factors against diseases associated with oxidative stress.