Polydimethylsiloxane Core-Polycaprolactone Shell Nanofibers as Biocompatible, Real-Time Oxygen Sensors.
Polydimethylsiloxane Core-Polycaprolactone Shell Nanofibers as Biocompatible, Real-Time Oxygen Sensors.
复制标题
DOI：
10.1016/j.snb.2013.10.084
复制
发表时间：
2014-03-01
期刊：
影响因子：
--
通讯作者：
Lannutti JJ
中科院分区：
文献类型：
--
作者：
Xue R;Behera P;Xu J;Viapiano MS;Lannutti JJ
在细胞水平上实时、连续监测局部氧含量对于癌细胞生物学和组织工程的研究都是可取的。在本文中，我们报告了通过静电纺丝成功制造含有氧敏感探针的聚二甲基硅氧烷（PDMS）纳米纤维，以及这些纤维作为气态氧和溶解氧的光学氧传感器的应用。聚己内酯 (PCL) 的保护“壳”层不仅在缓慢固化过程中保持 PDMS 的纤维形态，而且还提供了更具生物相容性的表面。该策略完善后，三(4,7-二苯基-1,10-菲咯啉)钌(II) (Ru(dpp)) 和八乙基卟啉铂 (PtOEP) 溶解在 PDMS 核心中，并建立了最终的传感性能。这些包含不同灵敏度探针的新型核-壳传感器在氧响应方面表现出轻微的变化，但均表现出出色的斯特恩-沃尔默线性度。部分由于纤维的多孔性质和PDMS优异的透氧性，新传感器的响应速度比以前的报告快-4-10倍（<0.5秒），比传统的基于二维薄膜的氧传感器快-4-10倍。这种核壳纤维很容易整合到标准细胞培养板或生物反应器中。还评估了这些基于纳米纤维的传感器的光稳定性。神经胶质瘤细胞系（CNS1、U251）和神经胶质瘤来源的原代细胞（GBM34）的培养显示生物学行为的差异可以忽略不计，这表明核心内存在的卟啉染料不具有强烈的细胞毒性作用。 PCL“壳”所表现出的生物相容性与 PDMS 核心优异的氧气透明度的独特组合使得这种特殊的传感平台有望在生物环境中进行传感。
Real-time, continuous monitoring of local oxygen contents at the cellular level is desirable both for the study of cancer cell biology and in tissue engineering. In this paper, we report the successful fabrication of polydimethylsiloxane (PDMS) nanofibers containing oxygen-sensitive probes by electrospinning and the applications of these fibers as optical oxygen sensors for both gaseous and dissolved oxygen. A protective ‘shell’ layer of polycaprolactone (PCL) not only maintains the fiber morphology of PDMS during the slow curing process but also provides more biocompatible surfaces. Once this strategy was perfected, tris(4,7-diphenyl-1,10-phenanthroline) ruthenium(II) (Ru(dpp)) and platinum octaethylporphyrin (PtOEP) were dissolved in the PDMS core and the resulting sensing performance established. These new core-shell sensors containing different sensitivity probes showed slight variations in oxygen response but all exhibited excellent Stern-Volmer linearity. Due in part to the porous nature of the fibers and the excellent oxygen permeability of PDMS, the new sensors show faster response (<0.5 s) −4–10 times faster than previous reports – than conventional 2D film-based oxygen sensors. Such core-shell fibers are readily integrated into standard cell culture plates or bioreactors. The photostability of these nanofiber-based sensors was also assessed. Culture of glioma cell lines (CNS1, U251) and glioma-derived primary cells (GBM34) revealed negligible differences in biological behavior suggesting that the presence of the porphyrin dyes within the core carries with it no strong cytotoxic effects. The unique combination of demonstrated biocompatibility due to the PCL ‘shell’ and the excellent oxygen transparency of the PDMS core makes this particular sensing platform promising for sensing in the context of biological environments.