Bioinspired membrane-based systems for a physical approach of cell organization and dynamics: usefulness and limitations.
Bioinspired membrane-based systems for a physical approach of cell organization and dynamics: usefulness and limitations.
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DOI：
10.1098/rsfs.2015.0038
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发表时间：
2015-08-06
期刊：
影响因子：
4.4
通讯作者：
Bassereau P
中科院分区：
文献类型：
--
作者：
Lagny TJ;Bassereau P
细胞膜位于每个细胞区室的外围并包围整个细胞，同时与大部分细胞成分相互作用，细胞膜参与细胞的大部分重要功能。生物学家长期以来一直致力于破译膜是如何组织的，它们如何有助于运输，运动，胞质分裂，细胞间通讯，信息传输等，使用自上而下的方法和更先进的技术。相比之下，物理学家已经开发了自下而上的方法和越来越复杂的最小模型膜系统，以建立解释细胞膜如何工作以及它们如何与蛋白质（例如细胞骨架）相互作用的通用模型。我们回顾了目前可用的不同模型膜系统，以及它们如何帮助破译细胞功能，但也列出了它们的局限性。模型膜系统也用于合成生物学，并且可以具有超出基础研究的潜在应用。我们讨论了复杂的体外膜系统在生物背景下的发展和技术应用之间可能的协同作用。还可以讨论的问题是：我们还可以用合成系统做什么，我们在哪里停止建立，哪些是替代解决方案？
Being at the periphery of each cell compartment and enclosing the entire cell while interacting with a large part of cell components, cell membranes participate in most of the cell's vital functions. Biologists have worked for a long time on deciphering how membranes are organized, how they contribute to trafficking, motility, cytokinesis, cell–cell communication, information transport, etc., using top-down approaches and always more advanced techniques. In contrast, physicists have developed bottom-up approaches and minimal model membrane systems of growing complexity in order to build up general models that explain how cell membranes work and how they interact with proteins, e.g. the cytoskeleton. We review the different model membrane systems that are currently available, and how they can help deciphering cell functioning, but also list their limitations. Model membrane systems are also used in synthetic biology and can have potential applications beyond basic research. We discuss the possible synergy between the development of complex in vitro membrane systems in a biological context and for technological applications. Questions that could also be discussed are: what can we still do with synthetic systems, where do we stop building up and which are the alternative solutions?