Mechanism of pyrogallol autoxidation and determination of superoxide dismutase enzyme activity
Mechanism of pyrogallol autoxidation and determination of superoxide dismutase enzyme activity
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DOI：
10.1016/s0302-4598(98)00072-5
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发表时间：
1998-03-01
期刊：
影响因子：
--
通讯作者：
Gao, XR
中科院分区：
文献类型：
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作者：
Gao, RM;Yuan, ZB;Gao, XR
在pH值7.87 ~ 9.10范围内，研究了邻苯三酚在EDTA或DETAPAC（二乙烯三胺五乙酸）存在下的自氧化反应。邻苯三酚在弱碱性溶液中与二氧反应生成几种中间产物，这些中间产物是电活性物质，可用电分析方法检测。本文重点研究了pH对邻苯三酚自氧化过程中产生的一些中间产物的自氧化速率的影响，得到了相对于SCE在-0.20、-0.96和-1.45 V下的敏感二阶导数阴极波。讨论了反应机理。本文还提出了一种简便的超氧化物歧化酶活性电分析方法。(C) 1998 Elsevier Science S.A.
The autoxidation of pyrogallol was investigated in the presence of EDTA or DETAPAC (diethylenetriaminepentaacetic acid) in the pH range 7.87 to 9.10. Pyrogallol reacts with dioxygen in weakly alkaline solutions to form several intermediate products which are electroactive substance and can be detected by electroanalytical methods. The focus here was putted on the effect of pH on the autoxidation rate of some intermediate products produced in pyrogallol autoxidation, which gives sensitive second-order derivative cathodic waves at -0.20, -0.96 and -1.45 V vs. SCE, respectively. Reaction mechanism was discussed. The paper also presented a convenient electroanalytical assay for superoxide dismutase enzyme activity. (C) 1998 Elsevier Science S.A.