ATP Sulfurylase is Essential for the Utilization of Sulfamate as a Sulfur Source in the Yeast Komagataella pastoris (syn. Pichia pastoris).
ATP Sulfurylase is Essential for the Utilization of Sulfamate as a Sulfur Source in the Yeast Komagataella pastoris (syn. Pichia pastoris).
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DOI：
10.1007/s00284-017-1276-0
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发表时间：
2017-09
影响因子：
2.6
通讯作者：
Linder T
中科院分区：
文献类型：
--
作者：
Linder T
甲基营养酵母Komagataella Pastoris(SYN.巴斯德毕赤酵母)是已知的为数不多的能够利用磺酸盐()作为硫源的酵母之一。导致磺胺酸分解代谢的生化途径尚未确定。本研究旨在研究磺胺酸分解代谢是否通过无机硫中间体硫酸盐()或亚硫酸盐()进行，然后将其同化并并入含硫氨基酸及其衍生物中。分别缺失了巴斯德酵母无机硫同化途径中的两个关键基因，并研究了每个缺失突变体对磺酸盐等硫源的利用能力。Met3基因(编码三磷酸腺苷-硫酰基酶)的缺失不影响在L蛋氨酸、亚硫酸盐、甲烷磺酸或牛磺酸上的生长，但完全消除了在硫酸盐、硫酸甲酯和氨基磺酸上的生长。MET5基因(编码亚硫酸盐还原酶β亚基的基因)的缺失使除L蛋氨酸外的所有硫源都无法生长。这些结果表明，氨基磺酸的分解代谢在其同化之前通过硫酸盐中间体进行。
The methylotrophic yeast Komagataella pastoris (syn. Pichia pastoris) is one of the few known yeasts that can utilize sulfamate () as a sulfur source. The biochemical pathway responsible for the catabolism of sulfamate has yet to be identified. The present study sought to investigate whether sulfamate catabolism proceeds through either of the inorganic sulfur intermediates sulfate () or sulfite () before its assimilation and subsequent incorporation into sulfur-containing amino acids and their derivatives. Two key genes in the K. pastoris inorganic sulfur assimilation pathway were deleted separately and the ability of each deletion mutant to utilize sulfamate and other selected sulfur sources was studied. Deletion of the MET3 gene (which encodes the enzyme ATP sulfurylase) did not affect growth on l-methionine, sulfite, methanesulfonate, or taurine but completely abolished growth on sulfate, methyl sulfate and sulfamate. Deletion of the MET5 gene (which encodes the β subunit of the enzyme sulfite reductase) abolished growth on all tested sulfur sources except l-methionine. These results suggest that the catabolism of sulfamate proceeds through a sulfate intermediate before its assimilation.
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