Elicitor and resistance-inducing activities of β-1,4 cellodextrins in grapevine, comparison with β-1,3 glucans and α-1,4 oligogalacturonides
Elicitor and resistance-inducing activities of β-1,4 cellodextrins in grapevine, comparison with β-1,3 glucans and α-1,4 oligogalacturonides
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DOI：
10.1093/jxb/erm008
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发表时间：
2007-01-01
影响因子：
6.9
通讯作者：
Baillieul, Fabienne
中科院分区：
文献类型：
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作者：
Aziz, Aziz;Gauthier, Adrien;Baillieul, Fabienne
纤维糊精 (CD) 是由 β-1,4 葡萄糖苷残基组成的水溶性纤维素衍生物，已被证明可在葡萄 (Vitis vinifera L.) 细胞中诱导多种防御反应。 CD 的较大寡聚体迅速诱导 H2O2 的瞬时产生和游离胞质钙的升高，随后导致编码苯丙素途径关键酶和发病机制相关 (PR) 蛋白的基因差异表达，并刺激几丁质酶和 β-1,3 葡聚糖酶活性。大多数这些防御反应也是由不同聚合度（DP）的线性β-1,3葡聚糖（pGlu）和α-1,4低聚半乳糖醛酸（OGA）诱导的，但与βGlu和OGA效应相比，CD诱导的一些反应的强度不同。此外，使用 H2O2 产生的脱敏测定表明，用 CD 处理的细胞对第二次使用 OGA 仍保持完全响应，这表明葡萄细胞对这些寡糖的感知方式不同。 CD、β Glu 或 OGA 均不会诱导 HSR 基因表达，也不会诱导细胞死亡。根据葡萄细胞中的诱导子活性，用灰霉病菌攻击的 CD 孵育的叶子也导致该疾病的显着减少。数据表明，CD 可以通过除 β Glu 和 OGA 之外的其他不同反应途径发挥作用。他们还强调了每种寡糖需要特定的 DID 来诱导防御反应。
Cellodextrins (CD), water-soluble derivatives of cellulose composed of beta-1,4 glucoside residues, have been shown to induce a variety of defence responses in grapevine (Vitis vinifera L.) cells. The larger oligomers of CD rapidly induced transient generation of H2O2 and elevation in free cytosolic calcium, followed by a differential expression of genes encoding key enzymes of the phenylpropanoid pathway and pathogenesis-related (PR) proteins as well as stimulation of chitinase and beta-1,3 glucanase activities. Most of these defence reactions were also induced by linear beta-1,3 glucans (pGlu) and alpha-1,4 oligogalacturonides (OGA) of different degree of polymerization (DP), but the intensity of some reactions induced by CD was different when compared with beta Glu and OGA effects. Moreover, desensitization assays using H2O2 production showed that cells treated with CD remained fully responsive to a second application of OGA, suggesting a different mode of perception of these oligosaccharides by grape cells. None of CD, beta Glu, or OGA induced HSR gene expression nor did they induce cell death. In accordance with elicitor activity in grapevine cells, CD-incubated leaves challenged with Botrytis cinerea also resulted in a significant reduction of the disease. Data suggest that CD could operate via other distinct reaction pathways than beta Glu and OGA. They also highlight the requirement of a specific DID for each oligosaccharide to induce the defence response.