GEOMETRIC DETERMINANTS OF CELL VIABILITY FOR 3D-PRINTED HOLLOW MICRONEEDLE ARRAY-MEDIATED DELIVERY.
GEOMETRIC DETERMINANTS OF CELL VIABILITY FOR 3D-PRINTED HOLLOW MICRONEEDLE ARRAY-MEDIATED DELIVERY.
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3D 打印空心微针阵列介导的细胞活力的几何决定因素。
DOI：
10.1109/mems58180.2024.10439381
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发表时间：
2024
期刊：
影响因子：
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通讯作者：
Sochol,RyanD
中科院分区：
文献类型：
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作者：
Sarker,Sunandita;Wang,Jinghui;Shah,ShreyA;Jewell,ChristopherM;Rand-Yadin,Kinneret;Janowski,Miroslaw;Walczak,Piotr;Liang,Yajie;Sochol,RyanD
各种新兴的生物医学应用和临床干预依赖于通过中空、高纵横比的微针递送活细胞的能力。最近，微针阵列（MNA）已经获得了越来越多的兴趣，由于药物输送的固有好处，然而，研究探索利用这种优势的细胞输送的潜力已经受到阻碍，由于在制造高纵横比的MNA适合于提供哺乳动物细胞的困难。为了绕过这些挑战，在这里我们利用并扩展了我们之前报道的混合增材制造（或“三维（3D）打印）策略，即，将“Vat Photopolymerization（VPP）”技术、“Liquid Crystal Display（LCD）”3D打印与“Two-Photon Direct Laser Writing（DLW）"技术相结合，3D打印出适用于细胞递送研究的中空MNA。具体来说，我们3D打印了四组650 μ m高的MNA，对应于25 μm，50 μm，75 μm和100 μm的针特定内径（ID），然后检查了这些MNA对树突状细胞（DC）和HEK 293细胞的递送后活力的影响。实验结果表明，25 µm-ID的情况导致两种细胞类型的MNA递送后细胞活力在统计学上显着降低;然而，针头特定ID ≥ 50 µm的MNA在统计学上无法区分，也无法区分传统的32 G单针，从而为MNA介导的细胞递送提供了重要的基准。
A wide range of emerging biomedical applications and clinical interventions rely on the ability to deliver living cells via hollow, high-aspect-ratio microneedles. Recently, microneedle arrays (MNA) have gained increasing interest due to inherent benefits for drug delivery; however, studies exploring the potential to harness such advantages for cell delivery have been impeded due to the difficulties in manufacturing high-aspect-ratio MNAs suitable for delivering mammalian cells. To bypass these challenges, here we leverage and extend our previously reported hybrid additive manufacturing (or "three-dimensional (3D) printing) strategy—i.e., the combined the "Vat Photopolymerization (VPP)" technique, "Liquid Crystal Display (LCD)" 3D printing with "Two-Photon Direct Laser Writing (DLW)"—to 3D print hollow MNAs that are suitable for cell delivery investigations. Specifically, we 3D printed four sets of 650 µm-tall MNAs corresponding to needle-specific inner diameters (IDs) of 25 µm, 50 µm, 75 µm, and 100 µm, and then examined the effects of these MNAs on the post-delivery viability of both dendritic cells (DCs) and HEK293 cells. Experimental results revealed that the 25 µm-ID case led to a statistically significant reduction in post-MNA-delivery cell viability for both cell types; however, MNAs with needle-specific IDs ≥ 50 µm were statistically indistinguishable from one another as well as conventional 32G single needles, thereby providing an important benchmark for MNA-mediated cell delivery.