CircRNA ZNF609 in peripheral blood leukocytes acts as a protective factor and a potential biomarker for coronary artery disease
CircRNA ZNF609 in peripheral blood leukocytes acts as a protective factor and a potential biomarker for coronary artery disease
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DOI：
10.21037/atm-19-4728
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发表时间：
2020-06-01
影响因子：
--
通讯作者：
Zheng, Fang
中科院分区：
文献类型：
--
作者：
Liang, Bin;Li, Menglan;Zheng, Fang
背景：环状RNA（circular RNA，circRNA）在冠状动脉疾病（coronary artery disease，CAD）中表达异常。由于其特殊的结构，circRNA有可能成为特异性和稳定的标记物。方法：采用真实的时间聚合酶链反应（RT-PCR）检测330例冠心病患者和209例正常对照外周血白细胞（PBLs）中circZNF 609的表达。采用斯皮尔曼相关分析、多因素回归分析、多因素Logistic回归分析和受试者工作特征曲线（ROC）分析。此外，在小鼠巨噬细胞RAW 264. 7中过表达circZNF 609以研究其对炎性细胞因子的影响。结果：冠心病患者外周血淋巴细胞中circZNF 609的表达水平较对照组明显降低（变化倍数为0.4133，P
Background: Circular RNAs (circRNAs) have been reported to aberrantly express in coronary artery disease (CAD). Due to their special structures, circRNAs have the potential to be specific and stable markers. We conducted this study to explore circZNF609's function in atherosclerosis and to evaluate its predictive values for CAD.Methods: About 330 CAD patients and 209 controls were enrolled and the expression of circZNF609 in peripheral blood leukocytes (PBLs) was detected by quantitative real time polymerase chain reaction (RT-PCR). Spearman correlation, multivariate regression, multivariate logistic regression and receiver operating characteristic curve (ROC) were performed. Moreover, circZNF609 was overexpressed in mice macrophage RAW264.7 to investigate its influence on inflammatory cytokines. Finally, bioinformatics analysis was executed to excavate the potential downstream pathway of circZNF609.Results: The expression level of circZNF609 in PBLs of CAD patients was significantly decreased compared with the controls (the fold changes of 0.4133, P