TETRODOTOXIN AND NEUROMUSCULAR TRANSMISSION
TETRODOTOXIN AND NEUROMUSCULAR TRANSMISSION
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DOI：
10.1098/rspb.1967.0010
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发表时间：
1967-01-01
期刊：
影响因子：
--
通讯作者：
MILEDI, R
中科院分区：
文献类型：
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作者：
KATZ, B;MILEDI, R
利用河豚毒素河鲀毒素(T.T.)消除神经和肌肉纤维中的冲动传播，并研究在此条件下神经肌肉接头的生理特性。在 T.T. 麻痹的肌肉中记录了正常频率和振幅的自发微型终板电位。局部施加电流对运动神经末梢的去极化仍然会导致微型终板电位（epps）频率的通常增加。当短暂的电流脉冲施加到神经末梢时，可以诱发epps，其大小随着电流的强度而变化。与正常的 epp 一样，这些响应由统计上不同数量的微型电位组成。当钙从浴液中除去时，反应就会失败。当以不同的时间间隔施加两个相同的脉冲时，会促进第二个 epp，类似于通常在成对神经冲动中观察到的情况。结论是，河豚毒素在阻断神经和肌肉的电兴奋的同时，不干扰神经末梢释放乙酰胆碱，也不干扰其对肌纤维的局部作用。
The puffer fish poison, tetrodotoxin (T. T.) was applied to eliminate impulse propagation in nerve and muscle fibre, and the physiological properties of the neuromuscular junction were studied under this condition. Spontaneous miniature end-plate potentials of normal frequency and amplitude were recorded in the T. T. -paralysed muscle. Depolarization of motor nerve endings by locally applied current still produces the usual increase in the frequency of minature end-plate potentials (epps). When brief current pulses are applied to the nerve endings epps can be evoked, whose size varies with the intensity of the current. The responses are composed, like normal epps, of a statistically varying number of miniature potentials. The response fails when calcium is removed from the bath. When two identical pulses are applied at varying intervals, facilitation of the second epp occurs, similar to that observed normally with pairs of nerve impulses. It is concluded that tetro-dotoxin while blocking electric excitation in nerve and muscle does not interefere with the release of acetylcholine from nerve endings nor with its local action on the muscle fibre.