Pulmonary artery banding alters the expression of Ca2+ transport proteins in the right atrium in rabbits
Pulmonary artery banding alters the expression of Ca2+ transport proteins in the right atrium in rabbits
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DOI：
10.1152/ajpheart.00026.2009
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发表时间：
2009-06-01
影响因子：
4.8
通讯作者：
Janssen, Paul M. L.
中科院分区：
文献类型：
--
作者：
Gupta, Subash C.;Varian, Kenneth D.;Janssen, Paul M. L.
Gupta SC, Varian KD, Bal NC, Abraham JL, Periasamy M, Janssen PM. Pulmonary artery banding alters the expression of Ca2+ transport proteins in the right atrium in rabbits. Am J Physiol Heart Circ Physiol 296: H1933-H1939, 2009. First published April 17, 2009; doi: 10.1152/ajpheart.00026.2009.- Following pulmonary artery banding (PAB), the contractile function of right ventricle diminishes over time. Subsequently, the right atrium (RA) has to contract against a higher afterload, but it is unknown to what extent ventricular dysfunction has an effect on the atrial contractility. We hypothesized that right ventricular pressure overload may have an affect on atrial contractility and Ca2+ transport protein expression. Therefore, we induced pressure overload of the right ventricle by PAB for 10 wk in rabbits and examined the changes in the expression of Ca2+ transport proteins in the atrium. We demonstrate that PAB significantly decreased the expression of sarco(endo) plasmic reticulum Ca2+-ATPase (Serca) 2a while expression of Na+/Ca2+ exchanger-1 was significantly upregulated in the RA but not in the left atria of rabbit hearts, indicating that pressure is the major trigger. A decrease in Serca2a expression was concomitant with a significant decrease in sarcolipin (SLN), possibly indicating a compensatory role of SLN. The decreased expression of SLN was unable to completely restore sarcoplasmic reticulum Ca2+ uptake function of Serca2a. Functional contractile assessments in isolated trabeculae showed no difference between PAB- and sham-operated rabbits at 1 Hz but displayed an enhanced force development at higher frequencies and in the presence of isoproterenol, while twitch timing was unaffected. Our results indicate that right ventricular mechanical overload due to PAB affects the expression of the Ca2+-handling proteins in the RA in rabbits.
