Genetic analysis of nuclear-cytoplasmic incompatibility in pea associated with cytoplasm of an accession of wild subspecies Pisum sativum subsp elatius (Bieb.) Schmahl.
Genetic analysis of nuclear-cytoplasmic incompatibility in pea associated with cytoplasm of an accession of wild subspecies Pisum sativum subsp elatius (Bieb.) Schmahl.
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DOI：
10.1007/s00122-008-0940-y
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发表时间：
2009-02-01
影响因子：
5.4
通讯作者：
Kosterin, Oleg E.
中科院分区：
文献类型：
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作者：
Bogdanova, Vera S.;Galieva, Elvira R.;Kosterin, Oleg E.
以野生豌豆（Pisum sativum subsp.）为材料，研究了豌豆核质不亲和性的遗传基础。elatius）登录号VIR 320。当该加入物用作与家养豌豆（Pisum sativum subsp. sativum）的F-1是高度不育的，并显示叶绿素缺乏、叶绿素杂色、小叶和托叶减少，而正反交产生的杂种看起来正常。以测定系WL 1238和VIR 320为亲本，通过亲和方向杂交建立了作图重组自交系群体。通过将作为花粉亲本的单个RIL植物与作为细胞质供体的VIR 320杂交并对每个F-1的主要冲突迹象进行评分来分析引起核-细胞质冲突的能力。它的结论是，两个不连锁的核基因参与所观察到的不相容性的遗传控制。其中一个基因，表示为Scs 1，是紧密连锁的PhlC基因的连锁群III和其他，表示为Scs 2，是紧密连锁的gp基因的连锁群V。在WL 1238中的两个基因的等位基因是显性的，似乎是致命的纯合条件下，在VIR 320细胞质背景。
The genetic basis of nuclear-cytoplasmic incompatibility was examined using the wild pea (Pisum sativum subsp. elatius) accession VIR320. When this accession is used as the female parent in crosses with domesticated peas (Pisum sativum subsp. sativum) the F-1 is highly sterile and displays chlorophyll deficiency, chlorophyll variegation, reduction of leaflets and stipulae while the reciprocal cross produces hybrids that appear normal. A mapping recombinant inbred line (RIL) population was established based on a cross in a compatible direction of a tester line WL1238 with VIR320. The ability to cause nuclear-cytoplasmic conflict was analysed by crossing individual RIL plants as pollen parents with VIR320 as donor of cytoplasm and scoring each F-1 for major signs of the conflict. It is concluded that two unlinked nuclear genes are involved in the genetic control of the observed incompatibility. One of the genes, denoted as Scs1, is closely linked to the PhlC gene on linkage group III and the other, denoted as Scs2, is closely linked to the gp gene on linkage group V. Alleles of both genes in WL1238 are dominant and appear to be lethal in the homozygous condition in the VIR320 cytoplasm background.