Strains of Bradyrhizobium barranii sp. nov. associated with legumes native to Canada are symbionts of soybeans and belong to different subspecies (subsp. barranii subsp. nov. and subsp. apii subsp. nov.) and symbiovars (sv. glycinearum and sv. septentrion
Strains of Bradyrhizobium barranii sp. nov. associated with legumes native to Canada are symbionts of soybeans and belong to different subspecies (subsp. barranii subsp. nov. and subsp. apii subsp. nov.) and symbiovars (sv. glycinearum and sv. septentrion
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巴兰慢生根瘤菌 (Bradyrhizobium barranii sp.) 菌株。
DOI：
10.1099/ijsem.0.005549
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发表时间：
2022
影响因子：
2.8
通讯作者：
Minamisawa Kiwamu
中科院分区：
文献类型：
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作者：
Bromfield Eden S. P.;Cloutier Sylvie;Wasai-Hara Sawa;Minamisawa Kiwamu
从大豆根瘤中分离出的4株菌株与加拿大豆科植物根区土壤接种后，被鉴定为由共生菌种glycine earum和septentrionale组成的一个新的缓生根瘤菌谱系。我们的目的是通过系统发育、基因组和表型分析来验证这些菌株的分类地位。多种系统发育分析，包括对51个全长核糖体蛋白亚基（rps）基因序列的分析，证实了新菌株位于一个高度支持的谱系中，与已命名的带有b的慢生根瘤菌不同。是最近的亲戚。基于数字DNA-DNA杂交和基因组母细胞距离系统发育的基因组和系统发育分析结果表明，与近亲型菌株相比，新菌株低于物种划分的既定阈值（70%）。此外，根据设定的阈值79%，将新菌株分为两个亚种群。菌株144S4T、323S2、15s5和38s55的基因组大小分别为11 399 526、11 474 152、10580853和10 530 141 bp， DNA G+C含量分别为63.1、63.0、63.4和63.3 mol%。这些菌株具有共生岛，其中包含关键的结瘤、固氮和III型分泌系统基因，以及丰富的插入序列，并在2至4个推定质粒之间。菌株144s4t和323S2 (sv。在与大豆的共生关系中，菌株1S5和38S5T的固氮效果较好。七片三片是无效的。形态学、生理学和共生特征的数据补充了基于序列的结果。本文提供的数据支持对一个新种和两个新亚种的描述，它们被命名为barraniisp慢生根瘤菌。11月subsp.barraniisubsp。11月(sv。甘氨酸菌)和巴拉氏缓生根瘤菌。11月subsp.apiisubsp。11月(sv。以菌株144S4T（=LMG 31552T=HAMBI 3722T）为种型菌株和barraniisubsp亚种的型菌株。菌株38S5T（=LMG 31556T=HAMBI 3721T）为apiisubsp亚种的型菌株。11月。
Four bacterial strains isolated from root nodules of soybean plants that had been inoculated with root-zone soils of legumes native to Canada were previously identified as a novelBradyrhizobiumlineage consisting of symbiovars (sv.) glycinearum and septentrionale. Our purpose was to verify the taxonomic status of these strains using phylogenetic, genomic and phenotypic analyses. Multiple phylogenetic analyses including analysis of 51 full-length ribosome protein subunit (rps) gene sequences confirmed placement of the novel strains in a highly supported lineage distinct from namedBradyrhizobiumspecies withB. japonicumUSDA 6Tas the closest relative. The results of genomic and phylogenomic analyses based on digital DNA–DNA hybridization and genomeblastdistance phylogeny showed that novel strains in comparisons with type strains of closest relatives were below the established threshold (70 %) for species delineation. Moreover, the novel strains were divided into two subspecies clusters based on the established threshold of 79 %. The genomes of strains 144S4T, 323S2, 1S5 and 38S5Thave sizes of 11 399 526, 11 474 152, 10580853 and 10 530 141 bp with DNA G+C contents of 63.1, 63.0, 63.4 and 63.3 mol%, respectively. These strains possess symbiosis islands harbouring key nodulation, nitrogen-fixation and type III secretion system genes as well as abundant insertion sequences and between two and four putative plasmids. Strains 144S4Tand 323S2 (sv. glycinearum) are effective with regard to nitrogen fixation in symbiotic association with soybeans whereas strains 1S5 and 38S5T(sv. septentrionale) are ineffective. Data for morphological, physiological and symbiotic characteristics complement the sequence-based results. The data presented here support the description of a new species and two new subspecies for which the namesBradyrhizobium barraniisp. nov. subsp.barraniisubsp. nov. (sv. glycinearum) andBradyrhizobium barraniisp. nov. subsp.apiisubsp. nov. (sv. septentrionale) are proposed with strain 144S4T(=LMG 31552T=HAMBI 3722T) as the species type strain and type strain of subsp.barraniisubsp. nov., and strain 38S5T(=LMG 31556T=HAMBI 3721T) as the type strain of subsp.apiisubsp. nov.