MEDIATION OF CYCLIC ADENOSINE-3',5'-MONOPHOSPHATE IN 1-METHYLADENINE PRODUCTION BY STARFISH OVARIAN FOLLICLE CELLS
MEDIATION OF CYCLIC ADENOSINE-3',5'-MONOPHOSPHATE IN 1-METHYLADENINE PRODUCTION BY STARFISH OVARIAN FOLLICLE CELLS
复制标题
DOI：
10.1016/0016-6480(89)90155-x
复制
发表时间：
1989-11-01
影响因子：
2.7
通讯作者：
NAGAHAMA, Y
中科院分区：
文献类型：
--
作者：
MITA, M;UETA, N;NAGAHAMA, Y
海星卵母细胞减数分裂的恢复是由卵巢卵泡细胞在性腺刺激物质（GSS）的影响下产生的1-甲基腺嘌呤（1-米德）诱导的。还报道了伴刀豆球蛋白A（ConA）和两种丝氨酸蛋白水解酶（胰蛋白酶和链霉蛋白酶）可以刺激1-米德的产生。本研究旨在确定这些化合物是否通过升高海星（Asterina pectinifera）卵泡细胞中的cAMP介导1-米德产生。浓度为0.1 mg/ml的GSS在卵泡细胞孵育1-2小时后显著刺激细胞外培养基中1-米德的积累。GSS还引起细胞内cAMP水平增加4 - 5倍。GSS的持续存在是维持cAMP和1-米德水平升高所必需的。细胞内cGMP的基础水平仅为cAMP的20%左右，并且不受GSS处理的影响。在1 mM，3-异丁基-1-甲基黄嘌呤（IBMX），一种有效的磷酸二酯酶抑制剂，刺激1-米德和cAMP的生产在浓度依赖性的方式。Con A和两种丝氨酸蛋白酶也提高了cAMP和1-米德的产生。Con A诱导的（1.0mg/ml）cAMP和1-米德的增加大于对GSS的响应（0.1mg/ml），并且被α-MeAde处理完全抑制。甲基-D-甘露糖苷（10 mM），Con A的竞争性抑制剂。这些结果有力地表明，cAMP是海星卵泡细胞产生1-米德的第二信使。
Resumption of meiosis in starfish oocytes is induced by 1-methyladenine (1-MeAde) produced by ovarian follicle cells under the influence of a gonad-stimulating substance (GSS). It has also been reported that concanavalin A (Con A) and two serine proteolytic enzyme (trypsin and Pronase) can stimulate 1-MeAde production. This study was undertaken to determine if 1-MeAde production induced by these compounds in mediated through elevation of cAMP in starfish (Asterina pectinifera) follicle cells. GSS at concentration of 0.1 mg/ml significantly stimulated 1-MeAde accumulation in extracellular medium after 1-2 hr of follicle cell incubations. GSS also caused a four- to fivefold increase in intracellular levels of cAMP. The continuous presence of GSS was required for the maintenance of elevated levels of cAMP and 1-MeAde. Basal levels of intracellular cGMP wee only about 20% fo those of cAMP and were not influenced by treatment with GSS. At 1 mM, 3-isobutyl-1-methylxanthine (IBMX), a potent phosphodiesterase inhibitor, stimulated both 1-MeAde and cAMP production in a concentration-dependent manner. Con A and two serine proteases also raised both cAMP and 1-MeAde production. Con A-induced (1.0 mg/ml) increased in cAMP and 1-MeAde were greater than the response to GSS (0.1 mg/ml) and were completely suppressed by treatment with .alpha.-methyl-D-mannoside (10 mM), a competitive inhibitor of Con A. These results strongly suggest that cAMP is a second messenger in the production of 1-MeAde by starfish ovarian follicle cells.