Conserved patterns of protein interaction in multiple species
Conserved patterns of protein interaction in multiple species
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DOI：
10.1073/pnas.0409522102
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发表时间：
2005-02-08
影响因子：
11.1
通讯作者：
Ideker, T
中科院分区：
文献类型：
--
作者：
Sharan, R;Suthram, S;Ideker, T
为了阐明全球范围内的细胞机制，我们对最近可用的秀丽隐杆线虫、果蝇和酿酒酵母的蛋白质-蛋白质相互作用网络进行了多重比较。这项比较整合了蛋白质相互作用和序列信息，揭示了所有三个物种中保守的 71 个网络区域，其中许多区域是后生动物独有的。我们利用这种保守性，发现了对 4,645 个先前未描述的蛋白质功能和 2,609 个先前未描述的蛋白质相互作用的统计显着支持。我们通过双杂交分析测试了 60 个酵母相互作用预测，证实了其中大约一半。值得注意的是，许多预测的功能和相互作用无法仅根据序列相似性来识别，这表明网络比较提供了超出从基因组收集的信息之外的重要生物信息。
To elucidate cellular machinery on a global scale, we performed a multiple comparison of the recently available protein-protein interaction networks of Caenorhabditis elegans, Drosophila melanogaster, and Saccharomyces cerevisiae. This comparison integrated protein interaction and sequence information to reveal 71 network regions that were conserved across all three species and many exclusive to the metazoans. We used this conservation, and found statistically significant support for 4,645 previously undescribed protein functions and 2,609 previously undescribed protein interactions. We tested 60 interaction predictions for yeast by two-hybrid analysis, confirming approximately half of these. Significantly, many of the predicted functions and interactions would not have been identified from sequence similarity alone, demonstrating that network comparisons provide essential biological information beyond what is gleaned from the genome.