THE EXPRESSION OF THE NA+ GLUCOSE COTRANSPORTER (SGLT1) GENE IN LAMB SMALL-INTESTINE DURING POSTNATAL-DEVELOPMENT
THE EXPRESSION OF THE NA+ GLUCOSE COTRANSPORTER (SGLT1) GENE IN LAMB SMALL-INTESTINE DURING POSTNATAL-DEVELOPMENT
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DOI：
10.1016/0005-2736(93)90357-6
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发表时间：
1993-03-14
期刊：
影响因子：
--
通讯作者：
SHIRAZIBEECHEY, SP
中科院分区：
文献类型：
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作者：
FREEMAN, TC;WOOD, IS;SHIRAZIBEECHEY, SP
我们先前已表明，在羔羊出生后的发育过程中，肠道钠/葡萄糖协同转运蛋白（SGLT1）的活性和数量急剧下降，并且通过向瘤胃羊的肠道腔内灌注D - 葡萄糖可使其恢复。本文所呈现的工作利用定量原位杂交组织化学技术，追踪了绵羊小肠在早期发育过程中沿纵轴和横轴的SGLT1基因表达情况。沿纵轴方向，SGLT1 mRNA首先在隐窝 - 绒毛连接处下方可被检测到，并迅速上升至该点上方约150微米处的峰值水平。达到最大值后，mRNA的量朝着绒毛顶端逐渐下降。这种沿隐窝 - 绒毛轴的mRNA积累模式在所有肠道位置和年龄组中均相似。沿小肠长度（横轴）方向，首先在远端肠道观察到SGLT1 mRNA水平下降。与取自小肠长度25%和50%处的组织相比，5周龄羔羊肠道（长度的75%处）中SGLT1 mRNA的这种下降是显著的（分别为P < 0.01和P < 0.02）。然而，在断奶期间观察到的该基因表达的下降与SGLT1的活性和数量的下降并不同步。在成年动物中，SGLT1的活性非常低，其mRNA的量也大大减少。这项工作支持了这样一个发现，即绵羊肠道在出生后的发育过程中，SGLT1的表达主要在转录后水平受到调控。
We have shown previously that the activity and abundance of the intestinal Na+/glucose cotransporter (SGLT1) declines dramatically during the postnatal development of lambs, and that it can be restored in the intestine of ruminant sheep by intra-luminal infusion Of D-glucose. The work presented in this paper has followed the expression of the SGLT1 gene along the vertical and horizontal axes of the ovine small intestine during early development, using quantitative in situ hybridisation histochemistry. Along the vertical axis, SGLT1 mRNA was first detectable just below the crypt-villus junction and rose rapidly to a peak level approx 150 mum above this point. After reaching a maximum, the amount of message gradually declined towards the villus tip. This pattern of mRNA accumulation along the crypt-villus axis was similar in all intestinal positions and age groups. Along the length of the small intestine (horizontal axis), a decline in the level of SGLT1 mRNA was observed first in the distal intestine. This decrease in SGLT1 mRNA was significant in the intestine (75% of length) of 5-week-old lambs when compared to tissue taken from 25 and 50% of length (P < 0.01 and P < 0.02, respectively). However, the observed fall in the expression of this gene during weaning did not coincide with the fall in activity and amount of SGLT1. In adult animals, where the activity of SGLT1 is very low, the amount of message was greatly reduced. This work supports the finding that the expression of SGLT1 is primarily controlled at the post-transcriptional level during the postnatal development of ovine intestine.