Mitotic and pheromone-specific intrinsic polarization cues interfere with gradient sensing in Saccharomyces cerevisiae.
Mitotic and pheromone-specific intrinsic polarization cues interfere with gradient sensing in Saccharomyces cerevisiae.
复制标题
有丝分裂和信息素特异性内在极化线索干扰酿酒酵母中的梯度传感。
DOI：
10.1073/pnas.1912505117
复制
发表时间：
2020
影响因子：
11.1
通讯作者：
Colman-Lerner,Alejandro
中科院分区：
文献类型：
--
作者：
Vasen,Gustavo;Dunayevich,Paula;Colman-Lerner,Alejandro
极性决定是许多过程的核心，包括有丝分裂和趋化性。在酿酒酵母中，出芽和交配投射（MP）的形成使用了一个重叠的系统皮质地标，收敛于小G蛋白Cdc 42。然而，信息素梯度感应必须覆盖Rsr 1依赖的内部极性线索用于萌芽。使用这个模型系统，我们问会发生什么时，内在和外在的空间线索不一致。是竞争还是合作？通过活细胞显微镜和微流体技术，我们发现了这个信号系统的三个以前被忽视的特征。首先，与运动相关的偏振片充当独立于所有已知线索的极性地标。第二，Rax 1-Rax 2复合物在细胞的远极中作为信息素促进的极性线索起作用。第三，内部线索在信息素梯度跟踪期间保持活跃，并且可以干扰该过程，使MP的位置偏置。酵母缺陷的内部线索利用对齐显着优于野生型与人工产生的信息素梯度。
Polarity decisions are central to many processes, including mitosis and chemotropism. InSaccharomyces cerevisiae, budding and mating projection (MP) formation use an overlapping system of cortical landmarks that converges on the small G protein Cdc42. However, pheromone-gradient sensing must override the Rsr1-dependent internal polarity cues used for budding. Using this model system, we asked what happens when intrinsic and extrinsic spatial cues are not aligned. Is there competition, or collaboration? By live-cell microscopy and microfluidics techniques, we uncovered three previously overlooked features of this signaling system. First, the cytokinesis-associated polarization patch serves as a polarity landmark independently of all known cues. Second, the Rax1-Rax2 complex functions as a pheromone-promoted polarity cue in the distal pole of the cells. Third, internal cues remain active during pheromone-gradient tracking and can interfere with this process, biasing the location of MPs. Yeast defective in internal-cue utilization align significantly better than wild type with artificially generated pheromone gradients.