Zinc supplementation decreases hepatic copper accumulation in LEC rat
Zinc supplementation decreases hepatic copper accumulation in LEC rat
复制标题
补充锌可降低 LEC 大鼠肝铜积累
DOI：
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发表时间：
2007
影响因子：
3.9
通讯作者：
M. Cherian
中科院分区：
文献类型：
--
作者：
Blanca P. Esparza Gonzalez;R. Fong;C. J. Gibson;I. Fuentealba;M. Cherian
以肝豆状核变性(WD)模型Long-Evans Cinnamon大鼠(LEC)为模型，研究了膳食锌对铜(Cu2+)诱导的肝损伤的影响。4周龄LEC(N=)和对照Long-Evans(LE)(N=32)雌性大鼠分为两组，一组喂以补锌饲料(I组)，另一组喂以普通啮齿动物饲料(II组)。LEC大鼠分别于6、8、10、12、18、20周龄处死；LE对照组大鼠于6、12、18、20周龄处死。饲喂锌饲料的LEC大鼠在6-18周龄时，肝脏中的铜浓度低于正常饲料喂养的LEC大鼠。I组LEC大鼠肝脏金属硫蛋白(MT)浓度在12~20周龄时升高，而II组LEC大鼠肝脏MT浓度在12周龄后下降。TUNEL法检测，各年龄段补充锌的LEC大鼠肝细胞凋亡率均降低。结果表明，补锌可降低LEC大鼠肝脏铜含量，延缓铜中毒的临床和病理变化。虽然实际的保护机制尚不清楚，但这可以解释为高锌含量导致的肠道MT对饲料中铜的隔离。
The effect of dietary zinc (Zn) supplementation on copper (Cu)-induced liver damage was investigated in Long-Evans Cinnamon rats (LEC), a model for Wilson's disease (WD). Four-week-old LEC (N=64) and control Long-Evans (LE) (N=32) female rats were divided into two groups; one group was fed with a Zn-supplemented diet (group I) and the other was given a normal rodent diet (group II). LEC rats were killed at 6, 8, 10, 12, 18, and 20 wk of age; the LE control rats were killed at 6, 12, 18, and 20 wk of age. Cu concentration in the liver was reduced in LEC rats fed the Zn-supplemented diet compared with LEC rats on the normal diet between 6 and 18 wk of age. Metallothionein (MT) concentration in the livers of LEC rats in group I increased between 12 and 20 wk of age, whereas hepatic MT concentration in LEC rats from group II decreased after 12 wk. Hepatocyte apoptosis, as determined by TUNEL, was reduced in Zn-supplemented LEC rats at all ages. Cholangiocellular carcinoma was observed only in LEC rats in group II at wk 20. These results suggest that Zn supplementation can reduce hepatic Cu concentration and delay the onset of clinical and pathological changes of Cu toxicity in LEC rats. Although the actual mechanism of protection is unknown, it could be explained by sequestration of dietary Cu by intestinal MT, induced by high dietary Zn content.
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DOI：
10.1093/jn/118.2.222
发表时间：
1988-02
期刊：
The Journal of nutrition
影响因子：
--
作者：
T. Blalock;M. Dunn;R. Cousins
通讯作者：
T. Blalock;M. Dunn;R. Cousins
影响因子：
3.8
作者：
Habeebu, SS;Liu, J;Klaassen, CD
通讯作者：
Klaassen, CD
DOI：
--
发表时间：
1998
期刊：
--
影响因子：
--
作者：
Steven R. Davis;Robert J. McMahon;Robert J. Cousins
通讯作者：
Steven R. Davis;Robert J. McMahon;Robert J. Cousins
用锌治疗威尔逊氏病：X.肠金属硫蛋白诱导。
DOI：
--
发表时间：
1992
期刊：
The Journal of laboratory and clinical medicine
影响因子：
--
作者：
Yuzbasiyan-Gurkan,V;Grider,A;Nostrant,T;Cousins,RJ;Brewer,GJ
通讯作者：
Brewer,GJ
影响因子：
4.2
作者：
COUSINS, RJ;LEEAMBROSE, LM
通讯作者：
LEEAMBROSE, LM