Proton magnetic resonance spectroscopic analysis of diadenosine 5',5"'-polyphosphates.
Proton magnetic resonance spectroscopic analysis of diadenosine 5',5"'-polyphosphates.
复制标题
二腺苷 5,5"-多磷酸盐的质子磁共振波谱分析。
DOI：
10.1016/0014-5793(90)80892-m
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发表时间：
1990
期刊：
影响因子：
3.5
通讯作者：
Puri,RN
中科院分区：
文献类型：
--
作者：
Mayo,KH;Mvele,OM;Puri,RN
Certain diadenosine 5′,5‴‐polyphosphates are potent inhibitors of ADP stimulated platelet aggregation, acting possibly via competitive ADP‐receptor binding.1H NMR studies of a series of such compounds where the number of phosphate groups between adenosine groups was varied from 2 to 6 were performed to analyze possible preferred solution conformations and to define structure‐activity relations. Relative to mononucleotides ADP and ATP, chemical shifts of adenosine proton resonances in diadenosine polyphosphate analogs are upfield shifted suggesting base stacking. This effect is greatest for AP2A and AP3A. Coupling constants of ribose ring proton resonances support the idea of an anti‐base‐ribose ring conformation, and3JH5'‐Pvalues suggest a preferred gauche H‐C‐O‐P structure. In all cases, NMR parameters for AP2A are near‐limiting values for a static base stacked conformation. Increasing the number of phosphate groups between adenosine moieties tends to weaken this interaction.
