Higher-order structure of mammalian chromatin deduced from viscoelastometry data.
Higher-order structure of mammalian chromatin deduced from viscoelastometry data.
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从粘弹性数据推导出哺乳动物染色质的高阶结构。
DOI：
10.1080/07391102.1999.10508387
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Lange,CS
中科院分区：
文献类型：
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作者：
Ostashevsky,JY;Reichman,B;Lange,CS
哺乳动物DNA的粘弹性测量（VE）的结果一直令人困惑，因为他们有两个数量级较小的测量粘弹性松弛时间的哺乳动物染色体比预期的染色体大小的DNA线性线圈。为了解决这个矛盾，我们应用了最近的G1染色体结构模型（J.Y. Ostashevsky，Mol Biol. Cell 9，3031-3040，1998），其中每条染色体的30 nm染色质纤维形成一串环簇（胶束）。该模型有两个参数：每个胶束的环数（f）和平均环尺寸（Mf），可以根据VE数据独立估计。使用我们的平台期V79中国仓鼠细胞的VE数据（未辐照和剂量高达40戈伊的X辐照），我们表明f-13，这是接近其他估计使用该模型（franges from 10-20），和Mf ≥ 2 Mbp，这与我们的类核数据（1.3 Mbp）和文献中使用各种技术（1-3 Mbp）所作的估计相似。
The results of viscoelastometry (VE) for mammalian DNA have been puzzling because they have two orders of magnitude smaller measured viscoelastic relaxation times for mammalian chromosomes than that expected for DNA linear coils of chromosomal size. In an attempt to resolve this discrepancy, we have applied a recent model of Gl chromosome structure (J.Y. Ostashevsky,Mol Biol. Cell 9, 3031–3040, 1998) in which the 30 nm chromatin fiber of each chromosome forms a string of loop clusters (micelles). This model has two parameters: the number of loops per micelle(f)and the average loop size(Mf), which can be estimated independently from VE data. Using our VE data for plateau phase V79 Chinese hamster cells (unirradiated and X-irradiated with doses up to 40 Gy) we show thatf-13, which is close to other estimates made using the model (franges from 10–20), and Mf∼ 2 Mbp, which is similar to estimates made from our nucleoid data (1.3 Mbp) and to estimates made in the literature using a variety of techniques (1–3 Mbp).