Systematic characterization of human prostatic fluid proteins with two-dimensional electrophoresis.
Systematic characterization of human prostatic fluid proteins with two-dimensional electrophoresis.
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通过二维电泳对人前列腺液蛋白进行系统表征。
DOI：
10.1093/clinchem/30.12.2026
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发表时间：
1984
影响因子：
9.3
通讯作者：
J. Grayhack
中科院分区：
文献类型：
--
作者：
Y. Tsai;H. Harrison;C. Lee;J. Daufeldt;L. Oliver;J. Grayhack
我们用二维凝胶电泳（ISO-DALT系统）对人类前列腺液进行了系统分析，并对正常和疾病相关的蛋白质模式进行了表征。通过对80名无前列腺病变的男性（年龄小于或等于50岁）收集的前列腺液进行分析，建立了前列腺液蛋白的参考图谱。前列腺液中与血清蛋白具有相同免疫原性的蛋白，采用参考池的亲和柱分离抗体和吸收（血清成分）和未吸收（非血清成分）部分的差异分析来鉴定。对30例患者（前列腺癌8例，前列腺炎合并良性前列腺增生10例，单纯良性前列腺增生6例，无症状慢性前列腺炎6例）的前列腺液中57种主要前列腺液蛋白的存在与否进行定性评分。有统计学意义的是，在这样评分的蛋白中，至少有8种蛋白发生了与疾病相关的改变。
We present a systematic analysis of human prostatic fluid with two-dimensional gel electrophoresis (the ISO-DALT system) and a characterization of normal and disease-related protein patterns. A reference map for prostatic fluid proteins was established by analysis of pooled prostatic fluids from 80 men (age less than or equal to 50 years) without prostatic lesions. Proteins in prostatic fluid that share immunogenicity with serum proteins were identified by use of antibody to whole human-serum protein in an affinity-column fractionation of a reference pool and differential analysis of the absorbed (serum components) and unabsorbed (non-serum components) fractions. Individual prostatic fluids from 30 patients (eight with prostatic cancer, 10 with prostatitis and benign prostatic hyperplasia, six with benign prostatic hyperplasia alone, and six with asymptomatic chronic prostatitis) were scored qualitatively with respect to the presence or absence of 57 major prostatic fluid proteins. Statistically significant, disease-correlated alterations were observed for at least eight of the proteins so scored.