Interaction of PLGA nanoparticles with human blood constituents
Interaction of PLGA nanoparticles with human blood constituents
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DOI：
10.1016/j.colsurfb.2004.05.007
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发表时间：
2005-02-10
影响因子：
5.8
通讯作者：
Morey, T
中科院分区：
文献类型：
--
作者：
Kim, D;El-Shall, H;Morey, T
当纳米粒子被注入血液用于药物递送或药物解毒时，必须避免这些粒子与血液成分发生有害的相互作用。在先前的研究中，人们研究了血浆中的白蛋白、免疫球蛋白G和纤维蛋白原在聚苯乙烯等疏水性聚合物模型上的吸附情况，以及表面疏水性降低的情况，表面疏水性的降低会导致乳胶粒子上吸附的蛋白质数量减少。然而，粒子对其他血液成分（如血液中的无机电解质）的摄取以及这些粒子在血流中的分散/凝聚特性尚未得到充分研究。最重要的是，这些粒子对血液凝固和溶血的影响尚不明确。在本研究中，采用纳米沉淀法合成了聚（乳酸 - 羟基乙酸）共聚物（PLGA）纳米粒子。通过使用不同的PLGA负载量以及PLGA纳米粒子与模拟血液（SBF）之间不同的接触时间，研究了从模拟血液中摄取血液电解质的情况以及纳米粒子在模拟血液中的稳定性（分散/聚集）。还通过测量原始PLGA、表面活性剂改性的PLGA和聚乙二醇化的PLGA对红细胞的溶血作用以及血液凝固情况，研究了粒子与血液有机成分的相互作用。（C）2004爱思唯尔公司。保留所有权利。
When nanoparticles are injected into the blood for drug delivery or drug detoxification, detrimental interaction of these particles with blood constituents must be avoided. In previous studies, the adsorption of albumin immunoglobulin G, and fibrinogen from blood plasma to a model hydrophobic polymer like polystyrene was investigated as was decreasing surface hydrophobicity, which quantitatively leads to decreasing amounts of adsorbed proteins on latex particles. However, the uptake of other blood constituents, such as inorganic blood electrolytes, by particles and the dispersion/coagulation characteristics of these particles in the blood stream have not been fully studied. Most importantly, the effects of these particles on blood coagulation and hemolysis are not well known.In the present study, the poly(lactide-co-glycolide) acid(PLGA) nanoparticles were synthesized by using nanoprecipitation. The uptake of blood electrolytes from simulated blood fluid (SBF) and the stability (dispersion/aggregation) of nanoparticles in SBF was examined by using different loading amounts of PLGA and different contact time between PLGA nanoparticles and SBF. The interaction of particles with the organic components of blood was also studied by using the measurement of red blood cell hemolysis and blood clotting with raw PLGA, surfactant modified PLGA, and PEGylated PLGA. (C) 2004 Elsevier B.V. All rights reserved.