Downregulation of selenium-binding protein 1 is associated with poor prognosis in lung squamous cell carcinoma.
Downregulation of selenium-binding protein 1 is associated with poor prognosis in lung squamous cell carcinoma.
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DOI：
10.1186/s12957-016-0832-6
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发表时间：
2016-03-08
影响因子：
3.2
通讯作者：
Zeng GQ
中科院分区：
文献类型：
--
作者：
Tan X;Liao L;Wan YP;Li MX;Chen SH;Mo WJ;Zhao QL;Huang LF;Zeng GQ
我们发现硒结合蛋白1（SBP 1）在人支气管上皮细胞致癌过程中逐渐减少。在永生化人支气管上皮细胞系16 HBE细胞中敲低SBP 1显著增加了B[a] P诱导的细胞转化效率。然而，SBP 1的表达与患者的临床病理因素之间的关系尚未完全确定。关于SBP 1在肺鳞状细胞癌预后中的作用尚不清楚。采用82例LSCC肺叶切除术患者的组织样本。采用免疫组化和蛋白质印迹法检测SBP 1蛋白的表达。分析SBP 1表达水平与患者临床病理特征的关系。采用考克斯比例风险回归分析和Kaplan-Meier法进行生存分析。喉鳞状细胞癌中SBP 1蛋白的表达明显低于相应的正常支气管上皮（NBE）组织（P = 0.000）。在喉鳞状细胞癌中，SBP 1的表达水平与患者的年龄、性别、吸烟状况、原发肿瘤分期（T）、TNM临床分期、远处转移（M）无关（P > 0.05）。而SBP 1表达下调与淋巴结转移率和总生存率显著相关（P < 0.05）。考克斯回归分析显示，SBP 1的低表达可能是影响喉鳞癌患者生存率的独立预后因素（P = 0.002）。结论：喉鳞癌发生发展过程中，SBP 1表达下调，可能在喉鳞癌发生发展中起重要作用。SBP 1可能是喉鳞癌一个新的潜在预后因子。
We found that selenium-binding protein 1 (SBP1) was progressively decreased in the human bronchial epithelial carcinogenic processes. Knockdown of SBP1 in immortalized human bronchial epithelial cell line 16HBE cells significantly increased the efficiency of B[a]P-induced cell transformation. However, the relationship between SBP1 expression and clinicopathological factors of patients has not been defined completely. The specific role of SBP1 in prognosis of lung squamous cell carcinoma (LSCC) is still unknown. Tissue samples from 82 patients treated by pulmonary lobectomy for LSCC were used. Immunohistochemistry and western blotting were used to detect the expressions of SBP1 protein. The relationships between the expression level of SBP1 and the clinicopathological features of patients were analyzed. Cox proportional hazard regression analysis and Kaplan–Meier method were used to perform survival analysis. Expressions of SBP1 proteins were significantly lower in LSCC tissues than that in the corresponding normal bronchial epithelium (NBE) tissues (P = 0.000). In LSCC, The expression levels of SBP1 had not correlated with patients’ age, gender, smoking state, primary tumor stages (T), TNM clinical stages, and distant metastasis (M) (P > 0.05). However, downregulation of SBP1 was significantly associated with higher lymph node metastasis and lower overall survival rate (P < 0.05). Cox regression analysis indicated low expressions of SBP1 can be an independent prognostic factor for poor overall survival in LSCC patients (P = 0.002). Downregulation of SBP1 may play a key role in the tumorigenic process of LSCC. SBP1 may be a novel potential prognostic factor of LSCC.