Comparison of a portable breath hydrogen analyser (Micro H2) with a Quintron MicroLyzer in measuring lactose maldigestion, and the evaluation of a Micro H2 for diagnosing hypolactasia
Comparison of a portable breath hydrogen analyser (Micro H2) with a Quintron MicroLyzer in measuring lactose maldigestion, and the evaluation of a Micro H2 for diagnosing hypolactasia
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DOI：
10.1080/00365519850186607
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发表时间：
1998-05-01
影响因子：
2.1
通讯作者：
Korpela, R
中科院分区：
文献类型：
--
作者：
Peuhkuri, K;Poussa, T;Korpela, R
呼出气中氢的测定以及血糖浓度和尿半乳糖排泄量的变化是诊断乳糖缺乏的间接方法。本研究的目的是比较便携式呼吸氢分析仪（Micro H-2）与广泛使用的模型（Quintron MicroLyzer），并将其与乳糖耐量试验中的血糖、尿半乳糖和胃肠道症状进行比较。在一夜禁食后，44名志愿者（18 - 66岁）单次口服50 g乳糖。用两种分析仪测量呼出气氢浓度的变化，并测定4 h血糖和尿半乳糖的变化，并用作参考。根据我们的黄金标准，18名受试者被诊断为消化不良，其中三项测试中至少有两项阳性：Quintron呼吸氢，血糖浓度和尿半乳糖排泄。呼气氢浓度相对于基线的最高增加是高度可变的：44 - 366 ppm（Micro H-2）或27 - 187 ppm（Quintron MicroLyzer）。与金标准相比，Micro H-2的灵敏度、特异性、阳性和阴性预测值分别为83%、96%、94%和89%。总体一致率为91%（95% CI 78 - 97%）。与Quintron相比，100%的病例（92 - 100%）诊断相同。因此，对于诊断乳糖缺乏症，Micro H-2在测量呼吸氢浓度方面与口服乳糖耐量试验中常用的Quintron MicroLyzer一样可靠。
The measurement of hydrogen in exhaled air and changes in the concentration of blood glucose and urine galactose excretion are indirect methods of diagnosing hypolactasia. The aim of this study was to compare a portable breath hydrogen analyser (Micro H-2) with a widely used model (Quintron MicroLyzer) and to compare them with the blood glucose, urine galactose, and gastrointestinal symptoms in the lactose tolerance test. After an overnight fast, 44 volunteers (18-66 y) ingested 50 g lactose in a single oral dose. Changes in exhaled breath hydrogen concentrations were measured with the two analysers, and changes in blood glucose and urinary galactose were assayed for 4 h and used as a reference. Eighteen subjects were diagnosed as maldigesters according to our gold standard of at least two positive tests out of the three: breath hydrogen by Quintron, blood glucose concentration, and urine galactose excretion. The highest increase in the breath hydrogen concentration over the baseline was highly variable: 44-366 ppm (Micro H-2) or 27-187 ppm (Quintron MicroLyzer). The sensitivity, specificity, and positive and negative predictive values of the Micro H-2 compared to the gold standard were 83%, 96%, 94% and 89%, respectively. Overall agreement was 91% (95% CI 78-97%). Compared to the Quintron, the diagnoses were identical in 100% of the cases (92-100%). Thus, for diagnosing hypolactasia, the Micro H-2 appeared as reliable for measuring breath hydrogen concentrations as Quintron MicroLyzer commonly used in oral lactose tolerance tests.