Oncolytic Sendai virus-induced tumor-specific immunoresponses suppress “simulated metastasis” of squamous cell carcinoma in an immunocompetent mouse model

Oncolytic Sendai virus-induced tumor-specific immunoresponses suppress “simulated metastasis” of squamous cell carcinoma in an immunocompetent mouse model
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溶瘤仙台病毒诱导的肿瘤特异性免疫反应抑制免疫活性小鼠模型中鳞状细胞癌的“模拟转移”

DOI：
10.1002/hed.25642
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发表时间：
2019
期刊：
Head & Neck
影响因子：
--
通讯作者：
Shiotani Akihiro
Shiotani Akihiro
中科院分区：
--
文献类型：
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作者：
Tanaka Yuya;Araki Koji;Tanaka Shingo;Miyagawa Yoshihiro;Suzuki Hiroshi;Kamide Daisuke;Tomifuji Masayuki;Uno Kosuke;Harada Eiko;Yamashita Taku;Ueda Yasuji;Inoue Makoto;Shiotani Akihiro

文献摘要

相似文献

本研究的目的是在BioKnife治疗原发性肿瘤后，验证uPA活性依赖的仙台病毒（BioKnife）的抗转移作用，并在模拟头颈部鳞状细胞癌（HNSCC）转移小鼠模型中分析其机制。方法采用小鼠HNSCC细胞系SCCVII建立小鼠转移模型。我们使用细胞毒性T淋巴细胞（CTL）测定、脾脏流式细胞术（FCM）和继发性肿瘤免疫组化（IHC）来评估肿瘤大小和肿瘤特异性免疫反应的诱导。结果BioKnife治疗组继发肿瘤明显缩小。BioKnife组CTL活性明显提高。流式细胞检查显示，BioKnife组树突状细胞和CD4+/CD8+淋巴细胞的诱导量显著增加。免疫组化显示CD8+淋巴细胞侵袭继发性肿瘤。结论BioKnife诱导的肿瘤特异性免疫反应是一种新型的、安全的、微创的控制和预防肿瘤转移的方法。
BackgroundThe objectives of this study were to demonstrate anti‐metastatic effect of BioKnife, uPA activity‐dependent oncolytic Sendai virus, after BioKnife treatment for primary tumor, and analyze its mechanisms in a simulated metastasis mouse model of head and neck squamous cell carcinoma (HNSCC).MethodsWe established a simulated metastasis mouse model using a murine HNSCC cell line “SCCVII.” We assessed a tumor size and an induction of tumor‐specific immunoresponses using cytotoxic T‐lymphocyte (CTL) assay, flow cytometry (FCM) in spleen and immunohistochemistry (IHC) in secondary tumor.ResultsSecondary tumors were significantly smaller in BioKnife‐treated group. CTL activities were significantly improved in BioKnife group. FCM revealed that induction of dendritic cells and CD4+/CD8+lymphocytes was significantly higher in BioKnife group. IHC showed that CD8+lymphocytes invaded secondary tumor.ConclusionTumor‐specific immunoresponses induced by BioKnife has great potential to be a novel, safe, and less invasive option for control and prevention of metastasis.