MONOCLONAL EPSTEIN-BARR-VIRUS GENOMES BUT LACK OF EBV-RELATED PROTEIN EXPRESSION IN DIFFERENT TYPES OF GASTRIC-CARCINOMA
MONOCLONAL EPSTEIN-BARR-VIRUS GENOMES BUT LACK OF EBV-RELATED PROTEIN EXPRESSION IN DIFFERENT TYPES OF GASTRIC-CARCINOMA
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DOI：
10.1111/j.1365-2559.1994.tb01350.x
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发表时间：
1994-10-01
期刊：
影响因子：
6.4
通讯作者：
MULLERHERMELINK, HK
中科院分区：
文献类型：
--
作者：
OTT, G;KIRCHNER, T;MULLERHERMELINK, HK
采用高度敏感的非放射性原位杂交技术，对39例胃癌切除标本进行了EB病毒RNA序列的检测。转录EBER序列被发现在七个（18%），包括4例未分化癌突出淋巴浸润和3个胃腺癌。在阳性肿瘤中，所有或几乎所有的肿瘤细胞核都被明显标记。在非异型增生上皮细胞和反应性炎症浸润中未检测到阳性信号。使用特异性探针的EBV可变串联重复区的克隆性分析产生了单一的附加带在所有四个测试的情况下，其中两个未分化癌和两个腺癌。通过免疫组织学，在原位或印迹试验中，阳性病例的肿瘤细胞中不存在EBV相关蛋白LMP或EBNA-2的表达。我们的研究结果表明，EB病毒感染胃癌发生在肿瘤发生的早期，但与鼻咽癌相反，不会导致EB病毒特异性蛋白的表达。
Thirty-nine resection specimens of gastric carcinomas have been investigated for the presence of EBV RNA sequences using a highly sensitive non-radioactive in situ hybridization technique. Transcribed EBER sequences were found in seven (18%), including four cases of undifferentiated carcinoma with prominent lymphoid infiltration and three gastric adenocarcinomas. In the positive tumours all, or nearly all, tumour nuclei were distinctly labelled. No positive signals could be detected in the non-dysplastic epithelial cells or the reactive inflammatory infiltrate. Clonality analysis using specific probes to the variable tandem repeat region of the EBV yielded single episomal bands in all four cases tested, two of which were undifferentiated carcinomas and two adenocarcinomas. By means of immunohistology, no expression of the EBV-related proteins LMP or EBNA-2 was present in tumour cells of positive cases in in situ or blotting attempts. Our results suggest that infection of gastric carcinomas by the EB virus occurs early in tumourigenesis but, in contrast to nasopharyngeal carcinomas, does not result in the expression of EBV-specific proteins.