Cell compartmentalisation in planctomycetes: novel types of structural organisation for the bacterial cell
Cell compartmentalisation in planctomycetes: novel types of structural organisation for the bacterial cell
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DOI：
10.1007/s002030100280
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发表时间：
2001-06-01
影响因子：
2.8
通讯作者：
Fuerst, JA
中科院分区：
文献类型：
--
作者：
Lindsay, MR;Webb, RI;Fuerst, JA
基于冷冻替代细胞薄片、冷冻断裂复制品的超微结构，研究了浮菌种 Pirellula marina、Isosphaera pallida、Gemmata obscuriglobus、Planctomyces mat-is 和“Candidatus Brocadia anammoxydans”的细胞组织，对于 Gemmata obscuriglobus 和 Pirellllla marina，根据序列进行计算机辅助 3-D 重建冷冻替代细胞的切片。所有浮霉菌细胞都显示出一个外围无核糖体区域，这里称为核糖体，围绕细胞的周边，以及一个内部区域，包括任何类核区域以及核糖体样颗粒，由单个胞质内膜（ICM）界定，在皮雷菌属物种中称为皮雷卢洛体。免疫金标记和核糖核酸酶-金细胞化学表明，在浮霉菌中，所有细胞 DNA 完全包含在由 ICM 界定的内部区域内，并且核质不包含 DNA，但至少包含一些细胞的 RNA。 Isosphaera pallida 和 Planctomyces mat-is 中的 ICM 内陷，使得细壁质在切片中形成细胞内部的主要部分，但在其他浮霉菌中，它仍然作为外围区域。在厌氧氨氧化（“anammox”过程）化能自养生物“Candidatus Brocadia anammoxydans”中，由 ICM 界定的内部区域包含另一个内部单膜界定区域，anam-moxosome。在 Gemmata obscuriglobus 中。内部 ICM 边界区域包含核体、一个双膜边界区域，其中包含细胞的核仁和除一些 RNA 之外的所有基因组 DNA。不同浮生菌的细胞区室化的共同特征与浮生菌作为细菌的不同分支的单系性质一致。浮霉菌细胞的共同组织计划构成了其他细菌细胞中未知的新类型。
The organisation of cells of the planctomycete species Pirellula marina, Isosphaera pallida, Gemmata obscuriglobus, Planctomyces mat-is and "Candidatus Brocadia anammoxidans" was investigated based on ultrastructure derived from thin-sections of cryosubstituted cells, freeze-fracture replicas, and in the case of Gemmata obscuriglobus and Pirellllla marina, computer-aided 3-D reconstructions from serial sections of cryosubstituted cells. All planctomycete cells display a peripheral ribosome-free region, termed here the paryphoplasm, surrounding the perimeter of the cell, and an interior region including any nucleoid regions as well as ribosome-like particles, bounded by a single intracytoplasmic membrane (ICM), and termed the pirellulosome in Pirellula species. Immunogold labelling and RNase-gold cytochemistry indicates that in planctomycetes all the cell DNA is contained wholly within the interior region bounded by the ICM, and the paryphoplasm contains no DNA but at least some of the cell's RNA. The ICM in Isosphaera pallida and Planctomyces mat-is is invaginated such that the paryphoplasm forms a major portion of the cell interior in sections, but in other planctomycetes it remains as a peripheral zone. In the anaerobic ammonium-oxidising ("anammox" process) chemoautotroph "Candidatus Brocadia anammoxidans" the interior region bounded by ICM contains a further internal single-membrane-bounded region, the anam-moxosome. In Gemmata obscuriglobus. the interior ICM-bounded region contains the nuclear body, a double-membrane-bounded region containing the cell's nucleoid and all genomic DNA in addition to some RNA. Shared features of cell compartmentalisation in different planctomycetes are consistent with the monophyletic nature of the planctomycetes as a distinct division of the Bacteria. The shared organisational plan for the planctomycete cell constitutes a new type not known in cells of other bacteria.