STRUCTURE OF THE REACTION CENTER FROM RHODOBACTER-SPHAEROIDES R-26 AND 2.4.1 - SYMMETRY-RELATIONS AND SEQUENCE COMPARISONS BETWEEN DIFFERENT SPECIES
STRUCTURE OF THE REACTION CENTER FROM RHODOBACTER-SPHAEROIDES R-26 AND 2.4.1 - SYMMETRY-RELATIONS AND SEQUENCE COMPARISONS BETWEEN DIFFERENT SPECIES
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DOI：
10.1073/pnas.85.23.9012
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发表时间：
1988-12-01
影响因子：
11.1
通讯作者：
FEHER, G
中科院分区：
文献类型：
--
作者：
KOMIYA, H;YEATES, TO;FEHER, G
紫色细菌的光合反应中心表现出近似的双对称轴，它与辅因子和L、M亚基有关。对于球形红杆菌的反应中心，从这个双重对称轴的偏差已经通过180度的叠加来量化。旋转，B支的辅因子到A支上M亚基到L亚基上。本文介绍了四种紫色细菌、一种绿色细菌的L和M亚基以及一种含光系统ii的绿藻的D1和D2亚基的序列比对。在所有六种中，与Rb结构有关的残基都是保守的。讨论了球状物及其在反应中心功能中的可能作用。提出了一种表征α曝光的方法。-基于保守残基周期性的膜螺旋。这种方法可能对膜蛋白的三维结构建模有用。
Photosynthetic reaction centers from purple bacteria exhibit an approximate twofold symmetry axis, which relates both the cofactors and the L and M subunits. For the reaction center from Rhodobacter sphaeroides, deviations from this twofold symmetry axis have been quantitated by superposing, by a 180.degree. rotation, the cofactors of the B branch onto the A branch and the M subunit onto the L subunit. An alignment of the sequences of the L and M subunits from four purple bacteria, one green bacterium, and the D1 and D2 subunits of a photosystem II-containing green alga is presented. The residues that are conserved in all six species are shown in relation to the structure of Rb. sphaeroides and their possible role in the function of the reaction center is discussed. A method is presented for characterizing the exposure of .alpha.-helices to the membrane based on the periodicity of conserved residues. This method may prove useful for modeling the three-dimensional structures of membrane proteins.