Limited rotational diffusion of DPH in human erythrocyte membranes.

Limited rotational diffusion of DPH in human erythrocyte membranes.
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DPH 在人红细胞膜中的旋转扩散有限。

DOI:
10.1016/0003-2697(78)90584-5
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发表时间:
1978
影响因子:
2.9
通讯作者:
P. Glatz
P. Glatz
中科院分区:
生物学4区
文献类型:
--
作者:
P. Glatz

文献摘要

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二苯己三烯(DPH)的旋转扩散决定了其荧光去极化。在有限旋转扩散理论的基础上,利用时间分辨偏振测量方法计算了该探针在人红细胞鬼膜中的扩散系数。37℃时扩散系数为5.9 × 107sec−1;将其与DPH在液体石蜡中的扩散系数进行比较,以估计膜双分子层的微粘度。
The rotational diffusion of diphenylhexatriene (DPH) determines its fluorescence depolarization. Time-resolved polarization measurements were used to calculate the coefficient of diffusion of this probe in human crythrocyte ghost membranes on the basis of a diffusion theory of limited rotation. The diffusion coefficient is 5.9 × 107sec−1at 37°C; this was compared with the diffusion coefficient of DPH in liquid paraffin for an estimation of the microviscosity of the membrane bilayer.