Quantitative metabolome analysis using capillary electrophoresis mass spectrometry
Quantitative metabolome analysis using capillary electrophoresis mass spectrometry
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DOI：
10.1021/pr034020m
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发表时间：
2003-09-01
影响因子：
4.4
通讯作者：
Nishioka, T
中科院分区：
文献类型：
--
作者：
Soga, T;Ohashi, Y;Nishioka, T
提出了一种毛细管电泳质谱（CE-MS）综合定量分析带电荷代谢物的新方法。代谢物首先根据电荷和大小通过CE分离，然后通过监测m/z值的大范围使用MS选择性检测。通过对枯草芽孢杆菌提取物中1692种代谢物的分析，证明了该方法的实用性，揭示了枯草芽孢杆菌产孢过程中代谢物的显著变化。
A new approach for the comprehensive and quantitative analysis of charged metabolites by capillary electrophoresis mass spectrometry (CE-MS) is proposed. Metabolites are first separated by CE based on charge and size and then selectively detected using MS by monitoring over a large range of m/z values. This method enabled the determination of 352 metabolic standards and its utility was demonstrated in the analysis of 1692 metabolites from Bacillus subtilis extracts, revealing significant changes in metabolites during B. subtilis sporulation.