Chemical modification of pure titanium surfaces to enhance the cytocompatibility and differentiation of human mesenchymal stem cells
Chemical modification of pure titanium surfaces to enhance the cytocompatibility and differentiation of human mesenchymal stem cells
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DOI：
10.4012/dmj.2018-257
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发表时间：
2019-12-01
影响因子：
2.5
通讯作者：
Furuichi, Yasushi
中科院分区：
文献类型：
--
作者：
Kado, Takashi;Aita, Hideki;Furuichi, Yasushi
本研究的目的是通过在钛种植体表面固定生物功能分子来提高人骨髓间充质干细胞在钛种植体表面的细胞相容性和分化能力。将Gly-Arg-Gly-Asp-Ser(GRGDS)多肽、人血浆纤维连接蛋白(PFN)或小牛皮I型胶原(Col)共价固定在钛表面。附着在COL和PFN固定的钛表面的细胞数量是附着在抛光的钛表面对照上的细胞数量的两倍。细胞的碱性磷酸酶活性以及矿化结节的形成，在CoL和PFN固定的钛表面上显著高于未抛光的表面。这些结果表明，生物功能分子如COL和PFN在钛表面的固定化增强了人骨髓间充质干细胞的附着、扩散、增殖和分化，从而可能导致更快的骨-钛结合。
The aim of this study was to improve the cytocompatibility and differentiation of human bone marrow-derived mesenchymal stem cells on the surface of titanium implants by immobilizing biofunctional molecules on their surface. Gly-Arg-Gly-Asp-Ser (GRGDS) peptides, human plasma fibronectin (pFN), or type I collagen from calf skin (Col) was covalently immobilized on the titanium surfaces. Twice as many cells attached to the Col- and pFN-immobilized titanium surfaces than attached to the as-polished surface control. The ALP activity of the cells, as well as the mineralized nodule formation, was significantly higher on the Col- and pFN-immobilized titanium surfaces than on the as-polished surfaces. These results indicate that the immobilization of biofunctional molecules such as Col and pFN on titanium surfaces enhances the attachment, spreading, proliferation, and differentiation of human bone marrow-derived mesenchymal stem cells, which may lead to a more rapid bone-titanium integration.