PHYSICAL MAPPING, CLONING, AND IDENTIFICATION OF GENES WITHIN A 500-KB REGION CONTAINING BRCA1
PHYSICAL MAPPING, CLONING, AND IDENTIFICATION OF GENES WITHIN A 500-KB REGION CONTAINING BRCA1
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DOI：
10.1073/pnas.92.10.4362
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发表时间：
1995-05-09
影响因子：
11.1
通讯作者：
SOLOMON, E
中科院分区：
文献类型：
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作者：
BROWN, MA;JONES, KA;SOLOMON, E
BRCA1是人类染色体17q21上的乳腺癌/卵巢癌易感基因。我们描述了包含 BRCA1 基因的 500 kb 基因组 DNA 区域的完整而详细的物理图谱以及噬菌体 P1 人工染色体中的部分克隆。从该区域分离出大约 70 个外显子，其中 11 个是 BRCA1 基因的组成部分。对其他外显子的分析揭示了 rho 相关 G 蛋白和干扰素诱导的亮氨酸拉链蛋白 IFP-35。
BRCA1 is a breast/ovarian cancer susceptibility gene on human chromosome 17q21. We describe a complete and detailed physical map of a 500-kb region of genomic DNA containing the BRCA1 gene and the partial cloning in phage P1 artificial chromosomes. Approximately 70 exons were isolated from this region, 11 of which were components of the BRCA1 gene. Analysis of the other exons revealed a rho-related G protein and the interferon-induced leucine-zipper protein IFP-35.