Label-free shotgun proteomics and metabolite analysis reveal a significant metabolic shift during citrus fruit development.
Label-free shotgun proteomics and metabolite analysis reveal a significant metabolic shift during citrus fruit development.
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DOI：
10.1093/jxb/err197
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发表时间：
2011-11
影响因子：
6.9
通讯作者：
Blumwald E
中科院分区：
文献类型：
--
作者：
Katz E;Boo KH;Kim HY;Eigenheer RA;Phinney BS;Shulaev V;Negre-Zakharov F;Sadka A;Blumwald E
采用无标记LC-MS/MS鸟枪蛋白质组学技术，对柑橘果实发育过程中蛋白质合成和代谢产物进行定量分析，研究柑橘果实发育过程中的代谢变化。我们的研究结果表明，发生在柑橘果实成熟过程中的代谢变化，其中的有机酸和氨基酸的积累，在发展的早期阶段转移到糖的合成在柑橘果实发育的后期阶段。转化酶的表达保持不变，而转化酶抑制剂的成熟上调。蔗糖-磷酸合成酶和蔗糖-6-磷酸磷酸酶的表达增加以及糖的快速积累表明，在果实发育后期，柑橘汁囊细胞也在合成蔗糖。
Label-free LC-MS/MS-based shot-gun proteomics was used to quantify the differential protein synthesis and metabolite profiling in order to assess metabolic changes during the development of citrus fruits. Our results suggested the occurrence of a metabolic change during citrus fruit maturation, where the organic acid and amino acid accumulation seen during the early stages of development shifted into sugar synthesis during the later stage of citrus fruit development. The expression of invertases remained unchanged, while an invertase inhibitor was up-regulated towards maturation. The increased expression of sucrose-phosphate synthase and sucrose-6-phosphate phosphatase and the rapid sugar accumulation suggest that sucrose is also being synthesized in citrus juice sac cells during the later stage of fruit development.