Ultrastructure of mitosis in the cowpea rust fungus Uromyces phaseoli var. Vignae.
Ultrastructure of mitosis in the cowpea rust fungus Uromyces phaseoli var. Vignae.
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coper牛豆状真菌乌莫罗司莫尔斯叶片中有丝分裂的超微结构。 vignae。
DOI：
10.1083/jcb.70.3.592
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发表时间：
1976-09
影响因子：
7.8
通讯作者：
Heath, M C
中科院分区：
文献类型：
--
作者：
Heath, I B;Heath, M C
描述了菜豆单胞锈菌（Uromyces phaseoli var. vignae）有丝分裂核的超微结构的各个方面，包括来自豇豆寄主中的胞间菌丝以及在体外诱导分化的侵染结构。间期与核相关的细胞器（NAO）由两个由嗜锇性棒连接的三层非圆形盘组成。当这些盘分离时，核内纺锤体在它们之间形成。纺锤体包含极间、相互交错、染色体和片段微管，它们排列形成一个中央束，中期染色体位于其表面。未发现中期板。每个动粒最多有三个微管，单倍体纺锤体上约有14条染色体。末期伸长似乎涉及极间微管的延伸，没有证据表明相互交错的微管仍然存在。同时，从每个NAO盘发育出许多细胞质微管，而在其他时期则很少或没有。间期NAO的重新形成涉及在末期后期形成一个香肠状的中间体。核膜保持完整，核仁在整个分裂过程中持续存在。纺锤体和NAO的各个方面似乎是子囊菌和高等担子菌之间的进化中间体，从而支持了担子菌从前者进化的理论，并表明有丝分裂特征与其他系统发育标记之间存在令人鼓舞的相关性。
Aspects of the ultrastructure of mitotic nuclei of the fungus Uromyces phaseoli var. vignae are described from both intercellular hyphae in the cowpea host and infection structures induced to differentiate in vitro. The interphase nucleus-associated organelle (NAO) consists of two trilamellar acircular disks connceted by an osmiophilic bar. The intranuclear spindle develops between these disks when they separate. The spindle contains pole to pole, interdigitating, chromosomal, and fragmentary microtubules arranged to form a central bundle along the surface of which lie the metaphase chromosomes. No metaphase plate is found. There are up to three microtubules per kinetochore and approximately 14 chromosomes on the haploid spindle. Telophase elongation appears to involve extension of pole to pole microtubules with no evidence for the remaining presence of interdigitating microtubules. Concomitantly, numerous cytoplasmic microtubules develop from each NAO disk where few or none are present in other phases. Reformation of the interphase NAO involves the formation of a sausage- shaped intermediate at late telophase. The nuclear envelope remains intact and the nucleolus persists throughtout division. Various aspects of the spindle and NAOs appear to be evolutionary intermediates between Ascomycetes and higher Basidiomycetes, thus supporting the theory of Basidiomycete evolution from the former group and demonstrating an encouraging correlation between mitotic characteristics and other phylogenetic markers.