Enhanced production of MMP-1, MMP-3, MMP-13, and RANTES by interaction of chondrocytes with autologous T cells
Enhanced production of MMP-1, MMP-3, MMP-13, and RANTES by interaction of chondrocytes with autologous T cells
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DOI：
10.1007/s00296-006-0116-5
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发表时间：
2006-09-01
影响因子：
4
通讯作者：
Nishioka, Kusuki
中科院分区：
文献类型：
--
作者：
Nakamura, Hiroshi;Tanaka, Michiaki;Nishioka, Kusuki
据报道，在骨关节炎（OA）中，T细胞和软骨细胞通过细胞表面分子如MHC、CD 4或CD 8相互作用，并且T细胞被激活。本研究的目的是探讨软骨细胞-T细胞相互作用的金属蛋白酶（MMP）和趋化因子的生产方面的反应。关节软骨和自体血液取自接受假体手术的OA和骨折患者。从OA患者收集滑液（SF）。将分离的软骨细胞与自体T细胞共培养。SF细胞用免疫组化染色或Alcian蓝染色进行分析。与使用Transwell的间接共培养相比，通过直接共培养，MMP-1、MMP-3、MMP-13和调节活化、正常T表达和分泌（RANTES）的产生增加。RANTES在OA中的产生率显著高于非关节炎样本。SF中可见CD 3阳性单个核细胞和软骨样细胞。软骨细胞-T细胞接触在OA样本中更具粘附性。这些结果表明，MMPs和RANTES的产生通过相互作用而增强，并且软骨细胞-T细胞接触在体内是可能的。
It has been reported that T cells and chondrocytes interact through cell surface molecules such as MHC, CD4 or CD8 in osteoarthritis (OA) and T cells are activated. The objective of this study is to investigate the responses of chondrocyte-T cell interaction in terms of metalloprotease (MMP) and chemokine production. Articular cartilage and autologous blood were obtained from patients with OA and fracture who under went prosthetic surgery. Synovial fluid (SF) was collected from OA patients. Isolated chondrocytes were co-cultured with autologous T cells. SF cells were analyzed by immunostaining or Alcian blue staining. The production of MMP-1, MMP-3, MMP-13, and regulated on activation, normal T expressed and secreted (RANTES) was enhanced by direct co-culture compared to indirect co-culture using Transwell. Production ratio of RANTES in OA was significantly higher than non-arthritic samples. CD3 positive mononuclear cells and chondrocyte-like cells were found in SF. Chondrocyte-T cell contact was more adhesive in OA samples. These results showed the production of MMPs and RANTES was enhanced by the interaction and that chondrocyte-T cell contact was possible in vivo.