Generation of furosine and color in infant/enteral formula-resembling systems
Generation of furosine and color in infant/enteral formula-resembling systems
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DOI：
10.1021/jf040088q
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发表时间：
2004-08-25
影响因子：
6.1
通讯作者：
Guerra-Hernández, E
中科院分区：
文献类型：
--
作者：
Rufían-Henares, JA;García-Villanova, B;Guerra-Hernández, E
通过测量婴儿和类似配方奶粉的模型体系中的呋喃和颜色形成，研究了美拉德反应的程度，这些模型体系是将酪氨酸钙、实验室获得的或商业乳清蛋白与乳糖或右旋麦芽糖(成分类似于婴儿和肠道配方奶粉生产中使用的成分)混合，并在100、120或140℃下加热0-30分钟制备的。用高效液相色谱法测定呋喃西林的含量，用反射光度计测L*、a*和b*的比色参数进行颜色测定。在美拉德反应的第一步之后，当初始成分具有较低的热损害时，可以测定呋喃。因此，在含有100毫克/100克蛋白质的成分中，呋喃酪氨酸可能是一个低热损伤的指标，在这些模型体系中使用的浓度，与婴儿和肠道配方中使用的浓度相似，乳糖中的呋喃氨基酸值(赖氨酸损失的间接测量)高于右旋麦芽糖体系，在该体系中，所有存在的糖中只有葡萄糖、麦芽糖、麦芽三糖和麦芽四糖显示出与酪蛋白的反应活性。最后，当初始成分具有较高的热损伤或模型体系在高温或长时间加热时，可以按照高级步骤进行颜色测定。在所测定的参数中，b*最敏感。
The extent of the Maillard reaction was studied by measuring furosine and color formation in infant and enteral formula-resembling model systems prepared by mixing calcium caseinate, laboratory-obtained or commercial whey protein with lactose or dextrinomaltose (ingredients similar to those used in infant and enteral formula manufacture) and heating the mixture at 100, 120, or 140degreesC for 0-30 min. The furosine determination was performed by HPLC and the color determination by measuring colorimetric parameters L*, a*, and b* in a reflection photometer. The first steps of the Maillard reaction could be followed by furosine determination when initial ingredients had low thermal damage. Hence, furosine may be an indicator of low thermal damage in ingredients with < 100 mg/100 g of protein, At the concentrations used in these model systems, similar to those in infant and enteral formulas, furosine values (indirect measure of lysine losses) were higher in lactose than in dextrinomaltose systems, in which only glucose, maltose, maltotriose, and maltotetraose among all of the sugars present showed reactivity with casein. Finally, the advanced steps could be followed by color determination when the initial ingredients had high thermal damage or the model systems were heated at high temperature or for a long time. Among the parameters assayed, b* was the most sensitive.