Lymphocyte subsets in healthy children from birth through 18 years of age: The pediatric AIDS clinical trials group P1009 study
Lymphocyte subsets in healthy children from birth through 18 years of age: The pediatric AIDS clinical trials group P1009 study
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DOI：
10.1016/j.jaci.2003.07.003
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发表时间：
2003-11-01
影响因子：
14.2
通讯作者：
Spector, SA
中科院分区：
文献类型：
--
作者：
Shearer, WT;Rosenblatt, HM;Spector, SA
背景资料：外周血淋巴细胞亚群需要在一个大的，城市，少数民族为主的健康儿童队列中确定，作为合适的对照受试者，用于解释这些细胞在几种疾病条件下的外观，特别是儿科HIV-1感染。目的：我们试图确定美国健康城市居住的婴儿，儿童和青少年中淋巴细胞亚群的分布。方法：在对807名从出生到18岁的未暴露于艾滋病毒的儿童进行的横断面研究中，通过三色流式细胞术测定了淋巴细胞亚群。细胞表面标记分析表明，年龄是24个淋巴细胞亚群分布的一个极其重要的变量，以百分比或绝对计数测量，例如，CD 4（辅助）T细胞，CD 8（细胞毒性）T细胞、CD 19 B细胞、CD 4 CD 45 RA CD 62 L（初始辅助）T细胞、CD 3 CD 4 CD 45 RO（记忆辅助）T细胞、CD 8 HLA-DR CD 38（活化的细胞毒性）T细胞和CD 8 CD 28（活化引发的细胞毒性）T细胞。检测实验室被证明是一个重要的变量，表明需要使用同一实验室或同一组实验室来检测一个人的血液，并检测对照人群和患病或接受治疗的人群。性别和种族，种族是不太重要的。结论：本研究的结果提供了一个控制人口的艾滋病毒感染的影响，评估正常发展和淋巴细胞亚群的分布在儿童的性别，所有种族，所有的种族背景，从出生到18岁的城市人口。这项研究的发现也将证明在解释许多其他慢性疾病，如原发性免疫缺陷，恶性肿瘤，类风湿性关节炎和哮喘儿童的免疫变化的宝贵。
Background: Peripheral blood lymphocyte subsets need to be determined in a large, urban, minority-predominant cohort of healthy children to serve as suitable control subjects for the interpretation of the appearance of these cells in several disease conditions, notably pediatric HIV-1 infection.Objective: We sought to determine the distribution of lymphocyte subsets in healthy urban-dwelling infants, children, and adolescents in the United States.Methods: Lymphocyte subsets were determined by means of 3-color flow cytometry in a cross-sectional study of 807 HIV-unexposed children from birth through 18 years of age.Results: Cell-surface marker analysis demonstrated that age was an extremely important variable in 24 lymphocyte subset distributions measured as percentages or absolute counts-eg, the CD4 (helper) T cell, CD8 (cytotoxic) T cell, CD19 B cell, CD4CD45RACD62L (naive helper) T cell, CD3CD4CD45RO (memory helper) T cell, CD8HLA-DRCD38 (activated cytotoxic) T cell, and CD8CD28 (activation primed cytotoxic) T cell. The testing laboratory proved to be an important variable, indicating the need for using the same laboratory or group of laboratories to assay an individual's blood over time and to assay control and ill or treated populations. Sex and race-ethnicity were much less important.Conclusion: The results of this study provide a control population for assessment of the effects of HIV infection on the normal development and distribution of lymphocyte subsets in children of both sexes, all races, and all ethnic backgrounds from birth through 18 years of age in an urban population. This study's findings will also prove invaluable in interpreting the immune changes in children with many other chronic diseases, such as primary immunodeficiency, malignancy, rheumatoid arthritis, and asthma.