Vestibular afferent projections to the brain stem in pigeons.
Vestibular afferent projections to the brain stem in pigeons.
复制标题
鸽子的前庭传入脑干的投射。
DOI：
10.1111/j.1749-6632.1996.tb15737.x
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发表时间：
1996
影响因子：
5.2
通讯作者：
Dickman,JD
中科院分区：
文献类型：
--
作者：
Dickman,JD
方法将动物麻醉（戊巴比妥），在无菌条件下通过手术暴露左侧迷路。分离出支配特定受体嵴或黄斑的前庭神经的单个分支，并紧邻受体神经上皮进行切割。将辣根过氧化物酶（HRP）或辣根过氧化物酶/霍乱毒素混合物（HRP-CTP）的游离晶体应用于切断的神经残端。接下来，将骨蜡涂抹在该区域上，缝合伤口，并施用镇痛剂（布托啡诺）。 48至96小时的存活期后，对动物给予过量麻醉剂并用肝素化盐水和乙醛固定剂经心灌注。在横向或旁矢状平面上切割冷冻的连续切片，然后使用二氨基联苯胺 (DAB) 或四甲基联苯胺 (TMB) 作为 HRP 和 HRP-CTP 示踪组织的显色剂进行组织化学处理。使用光学显微镜对传入纤维及其末端分布进行可视化和拍照，然后使用透明相机和数字键盘或使用重建软件在计算机屏幕上直接进行摄像机图像跟踪进行绘制以进行比较。为了描绘预期切断的神经分支和其他前庭器官之间可能的示踪剂扩散，对几只动物的受体神经上皮进行了检查，没有观察到示踪剂扩散的证据。
METHODSThe animals were anesthetized (Nembutal), and the left labyrinth was surgically exposed under aseptic conditions. A single branch of the vestibular nerve that innervated a specific receptor crista or macula was isolated and cut immediately adjacent to the receptor neuroepithelium. Free crystals of either horseradish peroxidase (HRP) or a horseradish peroxidase/cholera-toxin mixture (HRP-CTP) were applied to the cut nerve stump. Next, bone wax was applied over the area, the wound sutured closed, and analgesic (Butorphanol) administered. Following a survival period of 48 to 96 hours, the animals were given an overdose of anesthetic and perfused transcardially with heparinized saline and aldehyde fixatives. Frozen serial sections were cut in either the transverse or parasagittal planes, then processed histochemically using either diamino benzidine (DAB) or tetramethyl benzidine (TMB) as a chromagen for the HRP and HRP-CTP tracing tissue. Afferent fibers and their terminal distributions were visualized and photographed using light microscopy, then drawn for comparison using either camera lucida and a digipad or direct video camera image tracing on a computer screen with reconstruction software. For delineation of possible tracer spread between the intended cut nerve branch and other vestibular organs, the receptor neuroepithelia were examined in several animals, with no evidence of tracer spread being observed.