Membrane-bound lipoxygenase of rat cerebral microvessels.
Membrane-bound lipoxygenase of rat cerebral microvessels.
复制标题
大鼠脑微血管的膜结合脂氧合酶。
DOI：
10.1016/s0006-291x(85)80156-x
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发表时间：
1985
影响因子：
3.1
通讯作者：
H. Iwata
中科院分区：
文献类型：
--
作者：
A. Baba;M. Kimoto;T. Tatsuno;T. Inoue;H. Iwata
从大鼠大脑皮质分离的微血管具有花生四烯酸脂氧合酶活性，这不是由于血小板可能受到污染。主要产物经鉴定为12-羟基二十碳四烯酸。经过匀浆和超声处理的微血管制剂，脂氧合酶的活性被回收在膜和胞浆组分，而在血小板中的胞浆组分中被回收。微血管膜脂氧合酶对花生四烯酸的Km值为3.8 μM，约为血小板膜脂氧合酶的1/5。去甲二氢愈创木酸可抑制微血管脂氧合酶，而吲哚美辛则不抑制。
The microvessels isolated from rat cerebral cortex has arachidonate lipoxygenase activity, which was not due to possible contamination of the platelets. The major product was identified to be 12-hydroxyeicosatetraenoic acid. After homogenization and sonication of the microvessel preparations, the lipoxygenase activity was recovered both in the membrane- and the cytosolfractions, whereas that in the platelets was recovered in the cytosol fraction. Membrane-bound lipoxygenase of the microvessels has apparent Km value of 3.8 μM for arachidonic acid, which was corresponded to 1/5 of that in the platelet enzyme. Microvessel lipoxygenase was inhibited by nordihydroguaiaretic acid but not by indomethacin.
牛脑微血管的前列腺素内过氧化物代谢。
DOI：
10.1016/0005-2760(80)90068-5
发表时间：
1980
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Gerritsen,ME;Parks,TP;Printz,MP
通讯作者：
Printz,MP