Heterogeneous ceramide distributions alter spatially resolved growth of Staphylococcus aureus on human stratum corneum
Heterogeneous ceramide distributions alter spatially resolved growth of Staphylococcus aureus on human stratum corneum
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DOI：
10.1098/rsif.2017.0848
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发表时间：
2018-04-01
影响因子：
3.9
通讯作者：
German, Guy K.
中科院分区：
文献类型：
--
作者：
Cleary, Joseph M.;Lipsky, Zachary W.;German, Guy K.
当代研究揭示了健康和患病皮肤之间细菌微生物群多样性的巨大变化。然而，普遍使用拭子提取微生物意味着只能获得种群“快照”，而细菌生长的所有空间分辨信息都丢失了。在这里，我们报告的时空生长的金黄色葡萄球菌的表面上的人角质层（SC）;皮肤的最外层。这种细菌在患有特应性皮炎（AD）的皮肤上占优势。我们首先确定，神经酰胺的分布自然存在于SC是异质性的，并与组织的结构地形。这种分布随后影响细菌生物膜的生长。在SC中保持健康的神经酰胺浓度，生物膜表现出没有空间偏好的增长。相比之下，神经酰胺的消耗与已知的AD发生的减少一致，使得S。aureus使用地形峡谷的图案网络作为生长的管道。神经酰胺控制细菌生长的能力通过使用涂有神经酰胺亚组分D-鞘氨醇的地形皮肤峡谷类似物来证实。我们的工作似乎解释了神经酰胺消耗和S增加之间的因果关系。在AD中观察到的金黄色葡萄球菌群体。它还可以提供对疾病传播的深入了解，以及改善术前皮肤清洁技术。
Contemporary studies have revealed dramatic changes in the diversity of bacterial microbiota between healthy and diseased skin. However, the prevailing use of swabs to extract the microorganisms has meant that only population 'snapshots' are obtained, and all spatially resolved information of bacterial growth is lost. Here we report on the temporospatial growth of Staphylococcus aureus on the surface of the human stratum corneum (SC); the outermost layer of skin. This bacterial species dominates bacterial populations on skin with atopic dermatitis (AD). We first establish that the distribution of ceramides naturally present in the SC is heterogeneous, and correlates with the tissue's structural topography. This distribution subsequently impacts the growth of bacterial biofilms. In the SC retaining healthy ceramide concentrations, biofilms exhibit no spatial preference for growth. By contrast, a depletion of ceramides consistent with reductions known to occur with AD enables S. aureus to use the patterned network of topographical canyons as a conduit for growth. The ability of ceramides to govern bacterial growth is confirmed using a topographical skin canyon analogue coated with the ceramide subcomponent D-sphingosine. Our work appears to explain the causal link between ceramide depletion and increased S. aureus populations that is observed in AD. It may also provide insight into disease transmission as well as improving pre-operative skin cleansing techniques.