Elicitor-induced plant immunity relies on amino acids accumulation to delay the onset of bacterial virulence
Elicitor-induced plant immunity relies on amino acids accumulation to delay the onset of bacterial virulence
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诱导子诱导的植物免疫依赖于氨基酸积累来延迟细菌毒力的发生
DOI：
10.1093/plphys/kiad048
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发表时间：
2023
期刊：
影响因子：
7.4
通讯作者：
Danna, Cristian H
中科院分区：
文献类型：
--
作者：
Zhang, Xiaomu;Tubergen, Philip J;Agorsor, Israel D;Khadka, Pramod;Tembe, Connor;Denbow, Cynthia;Collakova, Eva;Pilot, Guillaume;Danna, Cristian H
植物免疫依赖于对入侵微生物的微生物相关分子模式（MAMPs）的感知，以诱导防御反应，抑制企图感染。已经提出mamp触发免疫（MTI）通过抑制细菌毒力的发作来抑制细菌感染。然而，人们对植物发挥这种作用的机制知之甚少。在这里，我们发现拟南芥（拟南芥）的MAMP感知以水杨酸（SA）依赖的方式诱导游离氨基酸的积累。当与谷氨酰胺和丝氨酸共浸润时，这两种氨基酸是由mamp诱导的高度积累的氨基酸，丁香假单胞菌。番茄DC3000表达了低水平的毒力基因，不能在其他易感植物中产生强大的感染。当外源性应用时，谷氨酰胺和丝氨酸直接抑制细菌的毒力和生长，绕过MAMP感知和SA信号。此外，谷氨酰胺dumper -1的功能获得突变体叶片外质体中内源性谷氨酰胺水平的增加挽救了部分受损的细菌毒力和生长抑制表型的thesa诱导缺陷-2 （sid2）突变体。我们的数据表明，MTI通过在感染的早期阶段通过过量的氨基酸延迟毒力的发作来抑制细菌感染。
Plant immunity relies on the perception of microbe-associated molecular patterns (MAMPs) from invading microbes to induce defense responses that suppress attempted infections. It has been proposed that MAMP-triggered immunity (MTI) suppresses bacterial infections by suppressing the onset of bacterial virulence. However, the mechanisms by which plants exert this action are poorly understood. Here, we showed that MAMP perception in Arabidopsis (Arabidopsis thaliana) induces the accumulation of free amino acids in a salicylic acid (SA)-dependent manner. When co-infiltrated with Glutamine and Serine, two of the MAMP-induced highly accumulating amino acids,Pseudomonas syringaepv. tomato DC3000 expressed low levels of virulence genes and failed to produce robust infections in otherwise susceptible plants. When applied exogenously, Glutamine and Serine directly suppressed bacterial virulence and growth, bypassing MAMP perception and SA signaling. In addition, an increased level of endogenous Glutamine in the leaf apoplast of a gain-of-function mutant ofGlutamine Dumper-1rescued the partially compromised bacterial virulence- and growth-suppressing phenotype of theSA-induced deficient-2 (sid2) mutant. Our data suggest that MTI suppresses bacterial infections by delaying the onset of virulence with an excess of amino acids at the early stages of infection.