A spectrophotometric study of the interaction of VO2+ with monophosphate nucleotides
A spectrophotometric study of the interaction of VO2+ with monophosphate nucleotides
复制标题
VO2 与单磷酸核苷酸相互作用的分光光度研究
DOI：
10.1016/0162-0134(93)80017-4
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
E. Baran
中科院分区：
文献类型：
--
作者：
P. A. Williams;E. Baran
用电子吸收光谱法研究了VO 2+与不同单磷酸核苷酸、D-核糖和D-核糖-5-磷酸的相互作用随pH的变化。该系统是高度pH值依赖性的，不同的配位球周围的金属中心，提高pH值。在pH = 11.0分光光度滴定表明形成2：1配体-金属络合物，其中VO 2+只与核糖部分的核苷酸相互作用。胸苷一磷酸是唯一研究的脱氧核糖核苷酸，与其他系统相比，表现出不同的行为。
The interaction of VO2+with different monophosphate nucleotides, as well as with D-ribose and D-ribose-5-phosphate, has been studied as a function of pH by electron absorption spectroscopy. The systems are highly pH-dependent, and different coordination spheres are generated around the metal center, with raising pH. Spectrophotometric titrations at pH = 11.0 demonstrates the formation of 2:1 ligand-to-metal complexes in which VO2+interacts only with the ribose moieties of the nucleotides. Thymidine monophosphate, the only investigated deoxyribonucleotide, shows a different behavior, in comparison to the other systems.