A CIS-ACTING ELEMENT FROM THE EPSTEIN-BARR VIRAL GENOME THAT PERMITS STABLE REPLICATION OF RECOMBINANT PLASMIDS IN LATENTLY INFECTED-CELLS
A CIS-ACTING ELEMENT FROM THE EPSTEIN-BARR VIRAL GENOME THAT PERMITS STABLE REPLICATION OF RECOMBINANT PLASMIDS IN LATENTLY INFECTED-CELLS
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DOI：
10.1073/pnas.81.12.3806
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
SUGDEN, B
中科院分区：
文献类型：
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作者：
YATES, J;WARREN, N;SUGDEN, B
apprx的EB病毒（EBV）基因组。在由病毒转化的人B淋巴母细胞中，170个腺苷酸酶对（kbp）作为质粒维持。在病毒基因组的1.8kbp内鉴定了顺式作用元件，其允许以高频率选择携带其的重组质粒并在潜伏感染EBV的细胞中维持为质粒。该功能元件需要至少800 bp且至多1800 bp长的DNA片段，其在一端含有30 bp串联重复序列家族。由于该区域仅对转染到含有EBV基因组的细胞中的质粒赋予有效的稳定复制，因此其功能可能需要在病毒基因组中其他地方编码的反式作用产物。
The Epstein-Barr viral (EBV) genome of .apprx. 170 kilobase pairs (kbp) is maintained as a plasmid in human B lymphoblasts transformed by the virus. A cis-acting element was identified within 1.8 kbp of the viral genome that allows recombinant plasmids carrying it to be selected at high frequency and maintained as plasmids in cells latently infected by EBV. This functional element(s) requires a segment of DNA at least 800 bp and at most 1800 bp long, which contains a family of 30 bp tandem repeats at 1 end. Since this region confers efficient stable replication only to plasmids transfected into cells containing EBV genomes, its function probably requires trans-acting products encoded elsewhere in the viral genome.