N‐myc suppresses major histocompatibility complex class I gene expression through down‐regulation of the p50 subunit of NF‐kappa B.
N‐myc suppresses major histocompatibility complex class I gene expression through down‐regulation of the p50 subunit of NF‐kappa B.
复制标题
N-myc 通过下调 NF-kappa B 的 p50 亚基来抑制主要组织相容性复合物 I 类基因表达。
作者：
Laura J. van't Veer;R. Beijersbergen;René Bernards
在神经母细胞瘤中，N-myc通过减少核因子与MHC I类基因启动子中增强子A元件的结合来抑制主要组织相容性复合体（MHC）I类抗原的表达。我们发现NF-κ B B的p50亚基是该复合物的一部分，并且N-myc抑制p50 mRNA的表达。在高水平表达N-myc的神经母细胞瘤细胞中转染p50表达载体，导致与MHC I类基因增强子结合的因子恢复，恢复增强子活性，并导致细胞表面MHC I类抗原的重新表达。这些数据表明NF-κ B B的p50亚基参与调节MHC I类抗原表达，并且N-myc主要通过抑制p50表达下调MHC I类基因表达。
In neuroblastoma, N‐myc suppresses the expression of major histocompatibility complex (MHC) Class I antigens by reducing the binding of a nuclear factor to the enhancer‐A element in the MHC Class I gene promoter. We show here that the p50 subunit of NF‐kappa B is part of this complex and that expression of p50 mRNA is suppressed by N‐myc. Transfection of a p50 expression vector in neuroblastoma cells that express N‐myc at a high level leads to restoration of factor binding to the MHC Class I gene enhancer, restores enhancer activity and leads to re‐expression of MHC Class I antigens at the cell surface. These data indicate that the p50 subunit of NF‐kappa B is involved in the regulation of MHC Class I antigen expression and that N‐myc down‐regulates MHC Class I gene expression primarily through suppression of p50 expression.